MICROPLATE READER-BASED QUANTITATION OF COLLAGENS

被引：109

作者：

WALSH, BJ ^{[1
]}

THORNTON, SC ^{[1
]}

PENNY, R ^{[1
]}

BREIT, SN ^{[1
]}

机构：

[1] UNIV NEW S WALES,SYDNEY,NSW,AUSTRALIA

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 203卷 / 02期

关键词：

Tissue culture - Hydroxyproline;

D O I：

10.1016/0003-2697(92)90301-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

By using a picrosirius dye, sensitive and specific staining of collagens plated in microtiter wells was achieved. The range of detection was from 0.5 to 20 μg. Human collagen types I, III, IV, and V were tested and able to be detected by the method. The dye did not bind to acetylcholinesterase or elastin. It did bind to C1q to some extent but this is not surprising since the molecule contains some triple helical collagen-like structures. A comparison performed between this assay and a colorimetric assay for hydroxyproline using tissue culture supernatants gave similar results for both samples. Due to its simplicity and sensitivity this assay will be most useful in laboratories where large numbers of samples must be screened for collagen production. © 1992.

引用

页码：187 / 190

页数：4

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