THE ACTIVITY OF PLASMINOGEN-ACTIVATOR INHIBITOR-1 (PAI-1) OF HUMAN PLATELET

被引：35

作者：

BOOTH, NA ^{[1
]}

CROLL, A ^{[1
]}

BENNETT, B ^{[1
]}

机构：

[1] UNIV ABERDEEN,DEPT MED & THERAPEUT,ABERDEEN AB9 1AS,SCOTLAND

来源：

FIBRINOLYSIS | 1990年 / 4卷

关键词：

PAI-1; Plasminogen activator inhibitor; Platelets;

D O I：

10.1016/0268-9499(90)90391-V

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have previously shown that platelets account for 93% of circulating PAI-1 antigen but for only half the PAI activity, suggesting that platelet PAI-1 is less active than plasma PAI-1. Here we show, both by an assay for the PAI-1 that is capable of forming a complex with t-PA and by a chromogenic assay for PAI activity, that human platelet PAI-1 is 3.5 ± 1.1% active. Human PAI-1, purified either from platelets or endothelial cells, was reactivated by 4M guanidine to a final specific activity of about 300,000 U/mg PAI-1. © 1990.

引用

页码：138 / 140

页数：3

共 9 条

[1] PLASMINOGEN ACTIVATORS IN ALCOHOLIC CIRRHOSIS - DEMONSTRATION OF INCREASED TISSUE-TYPE AND UROKINASE TYPE ACTIVATOR [J].

BOOTH, NA ;

ANDERSON, JA ;

BENNETT, B .

JOURNAL OF CLINICAL PATHOLOGY, 1984, 37 (07) :772-777

[2] PLASMINOGEN-ACTIVATOR INHIBITOR FROM HUMAN-ENDOTHELIAL CELLS - PURIFICATION AND PARTIAL CHARACTERIZATION [J].

BOOTH, NA ;

MACGREGOR, IR ;

HUNTER, NR ;

BENNETT, B .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 165 (03) :595-600

[3] PLATELET-RELEASE PROTEIN WHICH INHIBITS PLASMINOGEN ACTIVATORS [J].

BOOTH, NA ;

ANDERSON, JA ;

BENNETT, B .

JOURNAL OF CLINICAL PATHOLOGY, 1985, 38 (07) :825-830

[4] PLASMINOGEN-ACTIVATOR INHIBITOR (PAI-1) IN PLASMA AND PLATELETS [J].

BOOTH, NA ;

SIMPSON, AJ ;

CROLL, A ;

BENNETT, B ;

MACGREGOR, IR .

BRITISH JOURNAL OF HAEMATOLOGY, 1988, 70 (03) :327-333

[5] PLATELET PLASMINOGEN-ACTIVATOR INHIBITOR - PURIFICATION AND CHARACTERIZATION OF INTERACTION WITH PLASMINOGEN ACTIVATORS AND ACTIVATED PROTEIN-C [J].

FAY, WP ;

OWEN, WG .

BIOCHEMISTRY, 1989, 28 (14) :5773-5778

[6]

HEKMAN CM, 1985, J BIOL CHEM, V260, P11581

[7]

KRUITHOF E K O, 1988, Fibrinolysis, V2, P59

[8]

MACGREGOR I R, 1987, Fibrinolysis, V1, P247, DOI 10.1016/0268-9499(87)90044-0

[9]

SIMPSON AJ, 1990, IN PRESS BR J HAEMAT

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