BILIARY BILE-ACID PROFILES OF DOMESTIC-FOWL AS DETERMINED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND FAST ATOM BOMBARDMENT MASS-SPECTROMETRY

被引:15
作者
ELKIN, RG
WOOD, KV
HAGEY, LR
机构
[1] PURDUE UNIV,DEPT CHEM,W LAFAYETTE,IN 47907
[2] UNIV CALIF SAN DIEGO,DEPT MED,PHYSIOL PHARMACOL PROGRAM,LA JOLLA,CA 92093
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 96卷 / 01期
关键词
D O I
10.1016/0305-0491(90)90356-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. 1. The biliary bile acid profiles of domestic chickens (Gallus domesticus), turkeys (Meleagris gallopavo), and ducks (Anas platyrhynchos) were determiend by high performance liquid chromatography (HPLC) and fast atom bombardment mass spectrometry (FAB-MS). 2. 2. Chenodeoxycholyltaurine and cholyltaurine were the predominant bile acids in chicken and turkey bile, whereas duck bile contained primarily chenodeoxycholyltaurine and phocaecholyltaurine. 3. 3. Allocholyltaurine was also detected in chicken and turkey bile, but not in duck bile. 4. 4. FAB-MS analyses of individual HPLC peak fractions from chicken and duck bile extracts confirmed the presence of either taurine-conjugated dihydroxy- or trihydroxycholanoates. 5. 5. Direct FAB-MS analyses of avian bile extracts not subjected to HPLC permitted a rapid assessment of the relative proportion of taurine-conjugated dihydroxy- to trihydroxycholanoates. © 1990.
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页码:157 / 161
页数:5
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