MUTATIONAL ANALYSIS OF AN ESSENTIAL BINDING-SITE FOR THE U3 SNORNA IN THE 5' EXTERNAL TRANSCRIBED SPACER OF YEAST PRE-RIBOSOMAL-RNA

被引：33

作者：

BELTRAME, M ^{[1
]}

HENRY, Y ^{[1
]}

TOLLERVEY, D ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,D-69117 HEIDELBERG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 20期

关键词：

D O I：

10.1093/nar/22.20.4057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The small nucleolar RNA U3 is essential for viability in yeast. We have previously shown that U3 can be crosslinked in vivo to the pre-rRNA in the 5' external transcribed spacer (ETS), at +470. This ETS region contains 10 nucleotides of perfect complementarity to U3. In a genetic background where the mutated rDNA is the only transcribed rDNA repeat, the deletion of the 10 nt complementary to U3 is lethal. Cells lacking the U3 complementary sequence in pre-rRNA fail to accumulate 18S rRNA: pre-rRNA processing is inhibited at sites An in the 5' ETS, Al at the 5' end of 185 rRNA and A2 in ITS1. We show here that effects on processing at site A0 are specific for U3 and its associated proteins and are not seen on depletion of other snoRNP components. The deletion of the sequence complementary to U3 in the ETS therefore mimics all the known effects of the depletion of U3 in trans. This indicates that we have identified an essential U3 binding site on pre-rRNA, required in cis for the maturation of 18S rRNA.

引用

页码：4057 / 4065

页数：9

共 33 条

[1] MUTATIONAL ANALYSIS OF AN ESSENTIAL BINDING-SITE FOR THE U3 SNORNA IN THE 5' EXTERNAL TRANSCRIBED SPACER OF YEAST PRE-RIBOSOMAL-RNA (VOL 22, PG 4057, 1994)
BELTRAME, M
HENRY, Y
TOLLERVEY, D
NUCLEIC ACIDS RESEARCH, 1994, 22 (23) : 5138 - 5138
[2] U3 SMALL NUCLEAR-RNA CAN BE PSORALEN-CROSS-LINKED INVIVO TO THE 5' EXTERNAL TRANSCRIBED SPACER OF PRE-RIBOSOMAL-RNA
MASER, RL
CALVET, JP
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) : 6523 - 6527
[3] A NEW INTERACTION BETWEEN THE MOUSE 5' EXTERNAL TRANSCRIBED SPACER OF PRE-RIBOSOMAL-RNA AND U3 SNRNA DETECTED BY PSORALEN CROSS-LINKING
TYC, K
STEITZ, JA
NUCLEIC ACIDS RESEARCH, 1992, 20 (20) : 5375 - 5382
[4] DEPLETION OF U3 SMALL NUCLEOLAR RNA INHIBITS CLEAVAGE IN THE 5' EXTERNAL TRANSCRIBED SPACER OF YEAST PRE-RIBOSOMAL RNA AND IMPAIRS FORMATION OF 18S RIBOSOMAL-RNA
HUGHES, JMX
ARES, M
EMBO JOURNAL, 1991, 10 (13): : 4231 - 4239
[5] SECONDARY STRUCTURE OF THE 5' EXTERNAL TRANSCRIBED SPACER OF VERTEBRATE PRE-RIBOSOMAL-RNA - PRESENCE OF PHYLOGENETICALLY CONSERVED FEATURES
MICHOT, B
BACHELLERIE, JP
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 195 (03): : 601 - 609
[6] An evolutionary intra-molecular shift in the preferred U3 snoRNA binding site on pre-ribosomal RNA
Borovjagin, AV
Gerbi, SA
NUCLEIC ACIDS RESEARCH, 2005, 33 (15) : 4995 - 5005
[7] Distinct regions of U3 SnoRNA interact at two sites within the 5′ external transcribed spacer of pre-rRNAs in Trypanosoma brucei cells
Hartshorne, T
NUCLEIC ACIDS RESEARCH, 1998, 26 (11) : 2541 - 2553
[8] A U3 SMALL NUCLEAR RIBONUCLEOPROTEIN-REQUIRING PROCESSING EVENT IN THE 5' EXTERNAL TRANSCRIBED SPACER OF XENOPUS PRECURSOR RIBOSOMAL-RNA
MOUGEY, EB
PAPE, LK
SOLLNERWEBB, B
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (10) : 5990 - 5998
[9] INTRAGENIC PROCESSING IN YEAST RIBOSOMAL-RNA IS DEPENDENT ON THE 3' EXTERNAL TRANSCRIBED SPACER
MELEKHOVETS, YF
GOOD, L
ELELA, SA
NAZAR, RN
JOURNAL OF MOLECULAR BIOLOGY, 1994, 239 (02) : 170 - 180
[10] BASE-PAIRING BETWEEN U3 AND THE PRE-RIBOSOMAL-RNA IS REQUIRED FOR 18S RIBOSOMAL-RNA SYNTHESIS
BELTRAME, M
TOLLERVEY, D
EMBO JOURNAL, 1995, 14 (17): : 4350 - 4356

← 1 2 3 4 →