MASS RESOLUTION IMPROVEMENT BY INCORPORATION OF PULSED ION EXTRACTION IN A MATRIX-ASSISTED LASER-DESORPTION IONIZATION LINEAR TIME-OF-FLIGHT MASS-SPECTROMETER

被引:316
作者
BROWN, RS
LENNON, JJ
机构
[1] Department of Chemistry and Biochemistry, Utah State University, Logan, Utah
关键词
D O I
10.1021/ac00109a015
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A linear time-of-flight mass spectrometer has been modified to incorporate pulsed ion extraction of matrix-assisted laser desorption/ionization (MALDI) generated ions. A unique aspect of the experiments presented is the combination of pulsed extraction with very high source potentials (up to 25 kV) which allows improved mass resolution while maintaining excellent sensitivity for the large mit ions generated by the MALDI technique. Mass resolution in excess of 1000 (fwhm) is demonstrated for cytochrome c (12 361.1 Da) with the pulsed ion extraction linear time-of-flight mass spectrometer described. The influence on obtainable mass resolution of experimental variables such as delay time between laser ionization and ion extraction, amplitude of the pulsed voltage employed, and the source bias voltage are presented, It is shown that, for any given source potential, the optimum pulsed extraction voltage is a linear function of the mass of the analyte. This is consistent with the observation that the initial ion velocity distribution for MALDI-generated ions is independent of mass.
引用
收藏
页码:1998 / 2003
页数:6
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