RAPID METHOD, USING OCTADECASILYL-SILICA, FOR EXTRACTION OF CERTAIN PEPTIDES FROM TISSUES

被引:88
作者
BENNETT, HPJ [1 ]
HUDSON, AM [1 ]
KELLY, L [1 ]
MCMARTIN, C [1 ]
PURDON, GE [1 ]
机构
[1] CIBA GEIGY,CIBA LABS,HORSHAM RES CTR,DIV PHARMACEUT,WIMBLEHURST RD,HORSHAM RH12 4AB,W SUSSEX,ENGLAND
关键词
D O I
10.1042/bj1751139
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed by adsorption from the supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undamaged as determined by high-pressure liquid chromatography.
引用
收藏
页码:1139 / 1141
页数:3
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