AMINO ACID SEQUENCE OF FERREDOXIN FROM CLOSTRIDIUM ACIDI-URICI

The sequence of amino acids in Clostridium acidiurici ferredoxin was determined using two derivatives, S-β-aminoethylated ferredoxin and oxidized ferredoxin. Peptides derived from tryptic, papain, and partial acid hydrolyses were used to construct the total sequence. The sequence of amino acids in the individual peptides was determined by the Edman degradation and the exopeptidases leucine aminopeptidase, carboxypeptidase A, and carboxypeptidase B. Like the other two clostridial ferredoxins previously sequenced, C. acidiurici ferredoxin has an amino terminal alanine. Out of a total of 55, 37 amino acids are found in identical positions in all three species, including 8 cysteine and 3 proline residues. © 1969, American Chemical Society. All rights reserved.