MOLECULAR ANALYSIS AND EXPRESSION OF THE LIPASE OF STAPHYLOCOCCUS-EPIDERMIDIS

被引：68

作者：

FARRELL, AM ^{[1
]}

FOSTER, TJ ^{[1
]}

HOLLAND, KT ^{[1
]}

机构：

[1] UNIV DUBLIN TRINITY COLL, MOYNE INST, DEPT MICROBIOL, DUBLIN 2, IRELAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷

关键词：

D O I：

10.1099/00221287-139-2-267

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lipase of Staphylococcus epidermidis 9 was purified from culture supernatant fluid. Two polypeptides (51 and 43 kDa) were detected by SDS-PAGE, of which the 43 kDa polypeptide reacted with anti-lipase serum. The S. epidermidis 9 lipase gene (gehC) was cloned in Escherichia coli and localized to a 2.1 kb sequence by subcloning and transposon mutagenesis. The nucleotide sequence of gehC (2064 nucleotides) was determined and the predicted amino acid sequence of the encoded lipase (77 kDa) identified. A 97 kDa lipase was detected in extracts of E. coli harbouring gehC and in post-exponential-phase culture supernatant fluids of S. epidermidis 9. Data presented indicate that the lipase behaves anomalously during SDS-PAGE and that a pro-lipase is proteolytically processed in cultures of S. epidermidis 9 during growth.

引用

页码：267 / 277

页数：11

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