REGULATION OF LIPOGENESIS IN ADIPOCYTES - INDEPENDENT EFFECTS OF THYROID-HORMONES, CYCLIC-AMP AND INSULIN ON THE UPTAKE OF DEOXY-D-GLUCOSE

被引:20
作者
CORREZE, C [1 ]
KRUG, E [1 ]
VERHAEGEN, M [1 ]
NUNEZ, J [1 ]
机构
[1] INSERM, CNRS, UNITE RECH GLANDE THYROIDE & REGULAT HORMONALE, EQUIPE RECH 449, RUE DU GEN LECLERC, F-94270 BICETRE, FRANCE
关键词
(Adipocytes); Cyclic AMP; Deoxyglucose uptake; Lipogenesis; Thyroidectomy;
D O I
10.1016/0005-2760(79)90095-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Thyroidectomy enhances fat cell phosphodiesterase activity; as a result, the response to lipolytic hormones is markedly reduced. Thyroidectomy also stimulates overall lipogenesis and the uptake of glucose; the present experiments investigated whether there was a correlation between cyclic[c]AMP and glucose uptake. The parameter measured was the transport and phosphorylation (uptake) of deoxy-D-glucose in the presence of 2 modifiers of the cAMP pool: phosphodiesterase inhibitors and the analog, dibutyryl [db] cAMP. The inhibition by methylxanthines and dbcAMP of deoxy-D-glucose uptake observed, was the same in fat cells from normal and thyroidectomized rats; the latter still maintained their enhanced glucose uptake. Thyroid hormones and cAMP evidently control this step by different, separate pathways. Insulin, well known for its lipogenic effect, enhanced deoxy-D-glucose uptake in fat cells from both normal and thyroidectomized rats to the same extent (about 40%). An additive effect of thyroidectomy and insulin on glucose uptake was thus demonstrated. Glucose uptake in the adipocyte is controlled by at least 3 factors: thyroid hormones, cAMP and insulin, each of which can act independently. Maximum glucose uptake is achieved in the presence of a combination of low concentrations of cAMP, of insulin and in the absence of thyroid hormones.
引用
收藏
页码:164 / 172
页数:9
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