Following hypoglycemic injury to rat pheochromocytoma PC12 cells, cells display nuclear chromatin condensation and nucleosome-sized DNA fragmentation. Electrophoretic mobility shift assays were used to characterize binding of nuclear proteins to consensus sequences for AP-1, nuclear factor kappa B (NF kappa B), and octamer family after glucose deprivation. While AP-1 DNA binding activity and NF kappa B DNA binding activity were transiently stimulated, DNA binding to the octamer motif decreased. These data suggest that changes in nuclear protein binding to specific consensus sequences are an early molecular event in hypoglycemic-ischemic injury-induced neuronal cell death.
机构:Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, Israel
Haviv, R
Lindenboim, L
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机构:Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, Israel
Lindenboim, L
Yuan, JY
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机构:Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, Israel
Yuan, JY
Stein, R
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Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, IsraelTel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, Israel
机构:Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, Israel
Haviv, R
Lindenboim, L
论文数: 0引用数: 0
h-index: 0
机构:Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, Israel
Lindenboim, L
Yuan, JY
论文数: 0引用数: 0
h-index: 0
机构:Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, Israel
Yuan, JY
Stein, R
论文数: 0引用数: 0
h-index: 0
机构:
Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, IsraelTel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiochem, IL-69978 Ramat Aviv, Israel