CHARACTERIZATION OF THE INTERACTION OF ACETYLATED LDL AND OXIDATIVELY MODIFIED LDL WITH HUMAN LIVER PARENCHYMAL AND KUPFFER CELLS IN CULTURE

被引：34

作者：

KAMPS, JAAM ^{[1
]}

KRUIJT, JK ^{[1
]}

KUIPER, J ^{[1
]}

VANBERKEL, TJC ^{[1
]}

机构：

[1] LEIDEN UNIV,SYLVIUS LAB,CTR BIOPHARMACEUT SCI,DIV BIOPHARMACEUT,POB 9503,2300 RA LEIDEN,NETHERLANDS

来源：

ARTERIOSCLEROSIS AND THROMBOSIS | 1992年 / 12卷 / 09期

关键词：

OXIDATIVELY MODIFIED LOW DENSITY LIPOPROTEINS; ACETYLATED LOW DENSITY LIPOPROTEINS; SCAVENGER RECEPTORS; HUMAN LIVER PARENCHYMAL CELLS; HUMAN KUPFFER CELLS;

D O I：

10.1161/01.ATV.12.9.1079

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The interaction of acetylated low density lipoprotein (Ac-LDL) and oxidatively modified low density lipoprotein (Ox-LDL) with cultured human liver parenchymal cells and human Kupffer cells was investigated to define, for humans, the presence of scavenger receptors in the liver. A direct comparison of the capacity of Kupffer and parenchymal cells to interact with Ac-LDL and Ox-LDL indicated that the capacity of Kupffer cells per milligram of cell protein to degrade Ac-LDL and Ox-LDL is 14-fold and sixfold higher, respectively, than that of parenchymal cells. The degradation of both Ac-LDL and Ox-LDL by parenchymal cells and Kupffer cells could be inhibited by chloroquine and ammonium chloride, indicating that degradation occurs in the lysosomes. Competition studies showed that unlabeled Ox-LDL competed efficiently with the cell association and degradation of I-125-labeled Ac-LDL by human parenchymal cells and human Kupffer cells. However, unlabeled Ac-LDL did not compete (parenchymal cells) or only partially competed (40% in Kupffer cells) with the cell association and degradation of I-125-labeled Ox-LDL. Polyinosinic acid completely blocked the cell association and degradation of Ac-LDL and Ox-LDL with Kupffer cells while no significant effect on parenchymal cells was noted. It is concluded that human liver parenchymal cells contain a scavenger receptor that interacts with Ac-LDL and Ox-LDL and an additional recognition site that recognizes Ox-LDL specifically. On human Kupffer cells, as on rat Kupffer cells, in addition to the scavenger (Ac-LDL) receptor that interacts efficiently with both Ac-LDL and Ox-LDL, a specific Ox-LDL receptor is present at a relatively high concentration. The identification of various recognition sites for modified LDL on human liver cells indicates that the liver protection system against circulating modified LDL, identified earlier in rats, is also highly relevant for humans.

引用

页码：1079 / 1087

页数：9

共 38 条

[11] REGULATION OF LOW-DENSITY LIPOPROTEIN RECEPTOR ACTIVITY IN FRESHLY ISOLATED HUMAN LYMPHOCYTES [J].

HO, YK ;

BROWN, MS ;

BILHEIMER, DW ;

GOLDSTEIN, JL .

JOURNAL OF CLINICAL INVESTIGATION, 1976, 58 (06) :1465-1474

[12] UPTAKE AND DEGRADATION OF HUMAN LOW-DENSITY-LIPOPROTEIN BY HUMAN LIVER PARENCHYMAL AND KUPFFER CELLS IN CULTURE [J].

KAMPS, JAAM ;

KRUIJT, JK ;

KUIPER, J ;

VANBERKEL, TJC .

BIOCHEMICAL JOURNAL, 1991, 276 :135-140

[13] PROBUCOL PREVENTS THE PROGRESSION OF ATHEROSCLEROSIS IN WATANABE HERITABLE HYPERLIPIDEMIC RABBIT, AN ANIMAL-MODEL FOR FAMILIAL HYPERCHOLESTEROLEMIA [J].

KITA, T ;

NAGANO, Y ;

YOKODE, M ;

ISHII, K ;

KUME, N ;

OOSHIMA, A ;

YOSHIDA, H ;

KAWAI, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) :5928-5931

[14] VISUALIZATION OF THE INTERACTION OF NATIVE AND MODIFIED LIPOPROTEINS WITH PARENCHYMAL, ENDOTHELIAL AND KUPFFER CELLS FROM HUMAN LIVER [J].

KLEINHERENBRINKSTINS, MF ;

VANDEBOOM, JH ;

SCHOUTEN, D ;

ROHOLL, PJM ;

VANDERHEYDE, MN ;

BROUWER, A ;

VANBERKEL, TJC ;

KNOOK, DL .

HEPATOLOGY, 1991, 14 (01) :79-90

[15]

KMPS JAA, 1991, FEBS LETT, V287, P34

[16]

KOOISTRA T, 1989, THROMB HAEMOSTASIS, V62, P723

[17]

KRENNING EP, 1989, ENDOCRINOLOGY, V122, pT36

[18] RECEPTORS FOR MODIFIED LOW-DENSITY LIPOPROTEINS ON HUMAN ENDOTHELIAL-CELLS - DIFFERENT RECOGNITION FOR ACETYLATED LOW-DENSITY-LIPOPROTEIN AND OXIDIZED LOW-DENSITY-LIPOPROTEIN [J].

KUME, N ;

ARAI, H ;

KAWAI, C ;

KITA, T .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1091 (01) :63-67

[19] COMPARISON OF TAUROCHOLATE ACCUMULATION IN CULTURED-HEPATOCYTES OF PIG, RAT AND MAN [J].

KWEKKEBOOM, J ;

PRINCEN, HMG ;

VANVOORTHUIZEN, EM ;

MEIJER, P ;

KEMPEN, HJM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 162 (02) :619-625

[20]

LEMASTERS JJ, 1989, CELLS HEPATIC SINUSO, V2, P277

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