REGULATION OF THIAMINE BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE

被引：35

作者：

KAWASAKI, Y ^{[1
]}

NOSAKA, K ^{[1
]}

KANEKO, Y ^{[1
]}

NISHIMURA, H ^{[1
]}

IWASHIMA, A ^{[1
]}

机构：

[1] INST FERMENTAT,YODOGAWA KU,OSAKA 532,JAPAN

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 10期

关键词：

D O I：

10.1128/jb.172.10.6145-6147.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A pho6 mutant of Saccharomyces cerevisiae, lacking a regulatory gene for the synthesis of periplasmic thiamine-repressible acid phosphatase activity, was found to be auxotrophic for thiamine. The activities of four enzymes involved in the synthesis of thiamine monophosphate were hardly detectable in the crude extract from the pho6 mutant. On the other hand, the activities of these enzymes and thiamine-repressible acid phosphatase in a wild-type strain of S. cerevisiae, H42, decreased with the increase in the concentration of thiamine in yeast cells. These results suggest that thiamine synthesis in S. cerevisiae is subject to a positive regulatory gene, PHO6, whereas it is controlled negatively by the intracellular thiamine level.

引用

页码：6145 / 6147

页数：3

共 17 条

[1]

BROWN GM, 1970, METHODS ENZYMOLOGY A, V18, P162

[2]

CAMIENER GW, 1960, J BIOL CHEM, V235, P2411

[3] THE BIOSYNTHESIS OF THIAMINE AND THIAMINE PHOSPHATES BY EXTRACTS OF BAKERS YEAST [J].