PURIFICATION AND PROPERTIES OF NITROGENASE FROM THE CYANOBACTERIUM, ANABAENA-CYLINDRICA

被引：42

作者：

HALLENBECK, PC

KOSTEL, PJ

BENEMANN, JR

机构：

[1] Sanitary Engineering Research Laboratory, College of Engineering, University of California, Berkeley, Richmond, California, 94804

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 98卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb13186.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nitrogenase complex was isolated from nitrogen‐starved cultures of Anabaena cylindrica. Sodium dithionite, photochemically reduced ferredoxin, and NADPH were found to be effective electron donors to nitrogenase in crude extracts whereas hydrogen and pyruvate were not. The Km for acetylene in vivo is ten‐fold higher than the Kmin vitro, whereas this pattern does not hold for the non‐heterocystous cyanobacterium, Plectonema boryanum. This indicates that at least one mechanism of oxygen protection in vivo involves a gas diffusion barrier presented by the heterocyst cell wall. The Mo‐Fe component was purified to homogeneity. Its molecular weight (220000), subunit composition, isoelectric point (4.8), Mo, Fe, and S2− content (2, 20 and 20 mol/mol component), and amino acid composition indicate that this component has similar properties to Mo‐Fe‐containing components isolated from other bacterial sources. The isolated components from A. cylindrica were found to cross‐react, to varying degrees, with components isolated from Azotobacter vinelandii, Rhodospirillum rubrum, and P. boryanum. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：275 / 284

页数：10

共 42 条

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