ANALYSIS OF CHIRAL CARBOXYLIC-ACIDS IN WINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COUPLED UV AND CIRCULAR-DICHROISM DETECTION

被引：14
作者：
ZERBINATI, O
AIGOTTI, R
DANIELE, PG
机构：
[1] Dipartimento di Chimica Analitica, Università di Torino, I-10125 Turin
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 671卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(94)80251-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A circular dichroism (CD) spectrophotometer, equipped with a flow cell, and a UV detector were connected in series and were used for coupled UV-CD detection of optically active carboxylic acids. The limits of detection and the linear range for the enantiomers of tartaric, malic, lactic and ascorbic acids are reported. Several wines were analysed and CD detection proved to be sensitive enough for accurate determinations. The combined UV-CD detection was helpful for identifying the peaks and allowed the calculation of enantiomeric ratios.
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页码：281 / 285
页数：5
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