SIMILARITIES BETWEEN FUSOBACTERIUM-NUCLEATUM AND BACTEROIDES-FRAGILIS STUDIED BY 2 DNA PROBES DERIVED FROM FUSOBACTERIUM-NUCLEATUM

被引：0

作者：

BOLSTAD, AI

KLEIVDAL, H

JENSEN, HB

机构：

[1] UNIV BERGEN,DEPT BIOCHEM & MOLEC BIOL,BERGEN,NORWAY

[2] UNIV BERGEN,SCH DENT,ORAL MICROBIOL LAB,BERGEN,NORWAY

[3] UNIV BERGEN,GADE INST,DEPT MICROBIOL,BERGEN,NORWAY

来源：

SCANDINAVIAN JOURNAL OF DENTAL RESEARCH | 1994年 / 102卷 / 01期

关键词：

BACTEROIDES FRAGILIS; DNA PROBE; FUSOBACTERIUM NUCLEATUM; IMMUNOBLOTTING; POLYMERASE CHAIN REACTION; SDS-PAGE;

D O I：

暂无

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

A polymerase chain reaction (PCR)-amplified oligonucleotide DNA probe corresponding to a Fusobacterium nucleatum Fev1 DNA region coding for a 40-kDa major outer-membrane protein (OMP) and a randomly cloned 2.1 kb DNA probe were found to recognize DNA from the Gram-negative bacteria Fusobacterium nucleatum and Bacteroides fragilis on Southern blots and slot blots. The results:indicate sequence similarity within the DNA fragments studied. Immunobolts tested with polyclonal antibodies against whole cells of F. nucleatum revealed only weak antigen similarity between these species.

引用

页码：5 / 9

页数：5

共 20 条

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