STABILIZATION OF XYLANASE BY RANDOM MUTAGENESIS

被引：57

作者：

ARASE, A

YOMO, T

URABE, I

HATA, Y

KATSUBE, Y

OKADA, H

机构：

[1] OSAKA UNIV, FAC ENGN, DEPT BIOTECHNOL, 2-1 YAMADAOKA, SUITA, OSAKA 565, JAPAN

[2] KYOTO UNIV, INST CHEM RES, UJI, KYOTO 611, JAPAN

[3] OSAKA UNIV, INST PROTEIN RES, SUITA, OSAKA 565, JAPAN

来源：

FEBS LETTERS | 1993年 / 316卷 / 02期

关键词：

XYLANASE; BACILLUS-PUMILUS; RANDOM MUTAGENESIS; ENZYME STABILIZATION; HEAT-RESISTANT MUTANT;

D O I：

10.1016/0014-5793(93)81199-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four heat-resistant mutants of xylanase (N56, N102, N104 and F1) were obtained by random mutagenesis. The mutant genes had the following amino acid changes: N56, Ser-26 to Trp, Gly-38 to Asp and Thr-126 to Ser; N102, Gly-38 to Asp; N104, Gly-38 to Ser and Arg-48 to Lys; F1, Ser-12 to Cys. Kinetic studies showed that N104 is stabilized by an increase in the activation enthalpy, while the other mutants are stabilized by a decrease in the activation entropy.

引用

页码：123 / 127

页数：5

共 17 条

[1]

FELTON J, 1983, BIOTECHNIQUES, V1, P42

[2] THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE XYLANASE GENE (XYNA) OF BACILLUS-PUMILUS [J].