DIFFERENTIAL EXPRESSION OF CHITINASE AND BETA-1,3-GLUCANASE GENES IN VARIOUS TISSUES OF POTATO PLANTS

被引：0
|
作者：
GARCIAGARCIA, F
SCHMELZER, E
HAHLBROCK, K
ROXBY, R
机构：
[1] UNIV MAINE,DEPT BIOCHEM MICROBIOL & MOLEC BIOL,ORONO,ME 04469
[2] MAX PLANCK INST ZUCHTUNGSFORSCH,BIOCHEM ABT,D-50829 COLOGNE 30,GERMANY
来源：
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES | 1994年 / 49卷 / 3-4期
关键词：
SOLANUM TUBEROSUM; POLYSACCHARIDE METABOLISM; IN SITU MESSENGER-RNA HYBRIDIZATION; IMMUNOHISTOCHEMISTRY; PHENYLALANINE AMMONIA-LYASE;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have analyzed the tissue-specific expression of chitinase, beta-1,3-glucanase and, for comparison, phenylalanine ammonia-lyase genes in healthy, unstressed potato (Solanum tuberosum) plants by using RNA-RNA hybridization and immunostaining techniques to localize the respective mRNAs and proteins in situ. Preparations of leaf, newer, shoot tip, node and axillary bud revealed that chitinase exhibited a distinctive and characteristic expression pattern in being confined to the epidermal cell layer, while beta-1,3-glucanase and phenylalanine ammonia-lyase were present to varying extents in most tissues of these organs. These distinctive patterns of expression suggest fundamentally different roles for the two glucanohydrolases in the metabolism of unstressed plants.
引用
收藏
页码：195 / 203
页数：9
相关论文
共 50 条
  • [1] ACTIVITIES OF CHITINASE AND BETA-1,3-GLUCANASE IN SOIL
    JANDERA, A
    HANZLIKOVA, A
    SOTOLOVA, I
    FOLIA MICROBIOLOGICA, 1987, 32 (06) : 505 - 505
  • [2] Antifungal activity of cucumber beta-1,3-glucanase and chitinase
    Ji, C
    Kuc, J
    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1996, 49 (04) : 257 - 265
  • [3] SUPPRESSION OF BETA-1,3-GLUCANASE TRANSGENE EXPRESSION IN HOMOZYGOUS PLANTS
    DECARVALHO, F
    GHEYSEN, G
    KUSHNIR, S
    VANMONTAGU, M
    INZE, D
    CASTRESANA, C
    EMBO JOURNAL, 1992, 11 (07): : 2595 - 2602
  • [4] COORDINATED REGULATION OF CHITINASE AND BETA-1,3-GLUCANASE IN BEAN-LEAVES
    VOGELI, U
    MEINS, F
    BOLLER, T
    PLANTA, 1988, 174 (03) : 364 - 372
  • [5] STRUCTURE AND EXPRESSION OF A TOBACCO BETA-1,3-GLUCANASE GENE
    OHMETAKAGI, M
    SHINSHI, H
    PLANT MOLECULAR BIOLOGY, 1990, 15 (06) : 941 - 946
  • [6] Soybean roots infected by Glomus intraradices strains differing in infectivity exhibit differential chitinase and beta-1,3-glucanase expression
    Lambais, MR
    Mehdy, MC
    NEW PHYTOLOGIST, 1996, 134 (03) : 531 - 538
  • [7] Chitinase and beta-1,3-glucanase in the lutoid-body fraction of Hevea latex
    Subroto, T
    vanKoningsveld, GA
    Schreuder, HA
    Soedjanaatmadja, UMS
    Beintema, JJ
    PHYTOCHEMISTRY, 1996, 43 (01) : 29 - 37
  • [8] Stress-induced expression of cucumber chitinase and Nicotiana plumbaginifoliaβ-1,3-glucanase genes in transgenic potato plants
    Jana Moravčíková
    Jana Libantová
    Ján Heldák
    Ján Salaj
    Miroslav Bauer
    Ildikó Matušíková
    Zdenka Gálová
    Ľudmila Mlynárová
    Acta Physiologiae Plantarum, 2007, 29 : 133 - 141
  • [9] Stress-induced expression of cucumber chitinase and Nicotiana plumbaginifoliaβ-1,3-glucanase genes in transgenic potato plants
    Moravcikova, Jana
    Libantova, Jana
    Heldak, Jan
    Salaj, Jan
    Bauer, Miroslav
    Matusikova, Ildiko
    Galova, Zdenka
    Mlynarova, Ludmila
    ACTA PHYSIOLOGIAE PLANTARUM, 2007, 29 (02) : 133 - 141
  • [10] EXTRACELLULAR TARGETING OF THE VACUOLAR TOBACCO PROTEINS-AP24, CHITINASE AND BETA-1,3-GLUCANASE IN TRANSGENIC PLANTS
    MELCHERS, LS
    SELABUURLAGE, MB
    VLOEMANS, SA
    WOLOSHUK, CP
    VANROEKEL, JSC
    PEN, J
    VANDENELZEN, PJM
    CORNELISSEN, BJC
    PLANT MOLECULAR BIOLOGY, 1993, 21 (04) : 583 - 593
12345