SEPARATION OF POLYPEPTIDE-CHAINS OF RICIN AND THE INTERACTION OF THE A-CHAIN WITH CIBACRON BLUE-F3GA

被引：25
作者：
APPUKUTTAN, PS
BACHHAWAT, BK
机构：
[1] Indian Institute of Experimental Medicine, Calcutta
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 580卷 / 01期
关键词：
Cibacron Blue F[!sub]3[!/sub]GA; Galactomannan; Nucleotide-binding domain; Ricin polypeptide chain;
D O I：
10.1016/0005-2795(79)90192-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
From ricin bound to the galactomannan guar gum in a column, the nonbinding toxic A chain could be eluted by reduction with 2-mercaptoethanol and later the B chain by lactose. The presence of a nucleotide-binding domain on the toxic chain A could be demonstrated from its interaction with the blue dye Cibacron Blue F3GA. © 1979.
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页码：10 / 14
页数：5
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