ASSIGNMENT OF STRUCTURES TO OLIGOSACCHARIDES PRODUCED BY ENZYMATIC DEGRADATION OF A BETA-D-GLUCAN FROM BARLEY BY H-1-NMR AND C-13-NMR SPECTROSCOPY

被引：48

作者：

BOCK, K ^{[1
]}

DUUS, JO ^{[1
]}

NORMAN, B ^{[1
]}

PEDERSEN, S ^{[1
]}

机构：

[1] NOVO NORDISK A-S,DK-2880 BAGSVAERD,DENMARK

来源：

CARBOHYDRATE RESEARCH | 1991年 / 211卷 / 02期

关键词：

D O I：

10.1016/0008-6215(91)80093-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structures of one tri-(1), two tetra-(2 and 3), and one hexa-saccharide (4) produced by treatment of barley flour, after removal of the starch components, with a fungal beta-D-glucanase (Finizyme) have been assigned on the basis of H-1- and C-13-n.m.r. data as follows: beta-D-Glcp-(1 --> 3)-beta-D-Glcp-(1 --> 4)-D-Glcp (1), beta-D-Glcp-(1 --> 4)-beta-D-Glcp-(1 --> 3)-beta-D-Glcp-(1 --> 4)-D-Glcp (2), beta-D-Glcp-(1 --> 3)-beta-D-Glcp-(1 --> 4)-beta-D-Glcp-(1 --> 4)-D-Glcp (3), and beta-D-Xylp-(1 --> 4)-[alpha-L-Araf-(1 --> 3)]-[alpha-L-Araf-(1 --> 2)]-beta-D-Xylp-(1 --> 4)-beta-D-Xylp-(1 --> 4)-D-Xylp (4).

引用

页码：219 / 233

页数：15

共 34 条

[1] 2-DIMENSIONAL SPECTROSCOPY - APPLICATION TO NUCLEAR MAGNETIC-RESONANCE [J].