ASSIGNMENT OF STRUCTURES TO OLIGOSACCHARIDES PRODUCED BY ENZYMATIC DEGRADATION OF A BETA-D-GLUCAN FROM BARLEY BY H-1-NMR AND C-13-NMR SPECTROSCOPY

The structures of one tri-(1), two tetra-(2 and 3), and one hexa-saccharide (4) produced by treatment of barley flour, after removal of the starch components, with a fungal beta-D-glucanase (Finizyme) have been assigned on the basis of H-1- and C-13-n.m.r. data as follows: beta-D-Glcp-(1 --> 3)-beta-D-Glcp-(1 --> 4)-D-Glcp (1), beta-D-Glcp-(1 --> 4)-beta-D-Glcp-(1 --> 3)-beta-D-Glcp-(1 --> 4)-D-Glcp (2), beta-D-Glcp-(1 --> 3)-beta-D-Glcp-(1 --> 4)-beta-D-Glcp-(1 --> 4)-D-Glcp (3), and beta-D-Xylp-(1 --> 4)-[alpha-L-Araf-(1 --> 3)]-[alpha-L-Araf-(1 --> 2)]-beta-D-Xylp-(1 --> 4)-beta-D-Xylp-(1 --> 4)-D-Xylp (4).