COMPARISON OF DNA EXTRACTION AND AMPLIFICATION FROM ANCIENT HUMAN BONE AND MUMMIFIED SOFT-TISSUE

被引：34
作者：
LASSEN, C
HUMMEL, S
HERRMANN, B
机构：
[1] Institut für Anthropologie, Georg-August-Universität Göttingen, Göttingen, D-37073
来源：
INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 1994年 / 107卷 / 03期
关键词：
ANCIENT DNA; BONE; SOFT TISSUE AUTOMATED DNA EXTRACTION; PCR; SEX DETERMINATION;
D O I：
10.1007/BF01225603
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
South american precolumbian male mummies were employed as source material for a comparative investigation of bone and soft tissues by DNA analysis. The suitability of the DNA extracts from both sources was tested and evaluated by their effectiveness as target DNA in PCR amplifications. The results suggest that skeletal material should be given preference over soft tissues for PCR analysis if the material is severely degraded. This seems to be independent of the specific anatomical origin of the samples.
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页码：152 / 155
页数：4
相关论文
共 24 条
  • [1] CHA RS, 1993, PCR METH APPL, V3, P18
  • [2] CONE RW, 1993, PCR METH APPL, V3, P15
  • [3] Cooper A., 1993, ANCIENT DNA, P149
  • [4] Cooper A., 1992, ANCIENT DNA NEWSLETT, V1, P31
  • [5] ELLEGREN H, 1993, ANCIENT DNA RECOVERY, P211
  • [6] IDENTIFICATION OF THE REMAINS OF THE ROMANOV FAMILY BY DNA ANALYSIS
    GILL, P
    IVANOV, PL
    KIMPTON, C
    PIERCY, R
    BENSON, N
    TULLY, G
    EVETT, I
    HAGELBERG, E
    SULLIVAN, K
    [J]. NATURE GENETICS, 1994, 6 (02) : 130 - 135
  • [7] IDENTIFICATION OF THE SKELETAL REMAINS OF A MURDER VICTIM BY DNA ANALYSIS
    HAGELBERG, E
    GRAY, IC
    JEFFREYS, AJ
    [J]. NATURE, 1991, 352 (6334) : 427 - 429
  • [8] HAGELBERG E, 1993, ANCIENT DNA RECOVERY, P195
  • [9] HERRMANN B, 1993, ANCIENT DNA RECOVERY
  • [10] HOCHMEISTER MN, 1991, J FORENSIC SCI, V36, P1649
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