COMPARISON OF THE INHIBITION BY PHOSPHO(ENOL)PYRUVATE AND PHOSPHOGLYCOLATE OF PHOSPHOFRUCTOKINASE FROM BACILLUS-STEAROTHERMOPHILUS

被引:16
作者
TLAPAKSIMMONS, VL [1 ]
REINHART, GD [1 ]
机构
[1] UNIV OKLAHOMA,DEPT CHEM & BIOCHEM,NORMAN,OK 73019
关键词
D O I
10.1006/abbi.1994.1032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A comparison between the inhibition by phospho(enol)pyruvate (PEP) versus the inhibition by phosphoglycolate (PG) of phosphofructokinase (PFK) from Bacillus stearothermophilus is presented. Both inhibitors act by decreasing the apparent affinity displayed by the enzyme for its substrate fructose 6-phosphate (Fru-6-P) while having little effect on V(max). However, the two ligands differ in both their affinity for the enzyme and their effectiveness at antagonizing the subsequent binding of Fru-6-P. Although PG binds with approximately 10-fold lower affinity, it antagonizes the binding of Fru-6-P 3.5-fold more strongly than does PEP. Moreover, the enthalpy and entropy contributions to the coupling free energy between inhibitor and Fru-6-P, from which these antagonisms derive, reveal even greater differences between the ligands. These data indicate, therefore, that the changes in the structure of PFK from B. stearothermophilus that result from PG binding, which have been determined by X-ray crystallography (T. Schirmer and P.R. Evans, 1990 Nature 343, 140-145), may not be comparable to those that result from PEP binding and consequently do not represent the generic 'T-state,' as has been presumed. © 1994 Academic Press, Inc.
引用
收藏
页码:226 / 230
页数:5
相关论文
共 24 条
[1]   KINETICS OF ALLOSTERIC INTERACTIONS OF PHOSPHOFRUCTOKINASE FROM ESCHERICHIA COLI [J].
BLANGY, D ;
BUC, H ;
MONOD, J .
JOURNAL OF MOLECULAR BIOLOGY, 1968, 31 (01) :13-&
[2]  
BLOXHAM DP, 1973, ENZYMES, V8, P240
[3]   CRYSTALLOGRAPHIC STRUCTURE OF ALLOSTERICALLY INHIBITED PHOSPHOFRUCTOKINASE AT 7 A RESOLUTION [J].
EVANS, PR ;
FARRANTS, GW ;
LAWRENCE, MC .
JOURNAL OF MOLECULAR BIOLOGY, 1986, 191 (04) :713-720
[4]   STRUCTURE AND CONTROL OF PHOSPHOFRUCTOKINASE FROM BACILLUS-STEAROTHERMOPHILUS [J].
EVANS, PR ;
HUDSON, PJ .
NATURE, 1979, 279 (5713) :500-504
[5]   PHOSPHOFRUCTOKINASE - STRUCTURE AND CONTROL [J].
EVANS, PR ;
FARRANTS, GW ;
HUDSON, PJ .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1981, 293 (1063) :53-62
[6]   NUCLEOTIDE-SEQUENCE OF THE PHOSPHOFRUCTOKINASE GENE FROM BACILLUS-STEAROTHERMOPHILUS AND COMPARISON WITH THE HOMOLOGOUS ESCHERICHIA-COLI GENE [J].
FRENCH, BA ;
CHANG, SH .
GENE, 1987, 54 (01) :65-71
[7]   NUCLEOTIDE-SEQUENCE AND HIGH-LEVEL EXPRESSION OF THE MAJOR ESCHERICHIA-COLI PHOSPHOFRUCTOKINASE [J].
HELLINGA, HW ;
EVANS, PR .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 149 (02) :363-373
[8]   PURIFICATION BY AFFINITY CHROMATOGRAPHY, PROPERTIES AND CRYSTALLIZATION OF PHOSPHOFRUCTOKINASE FROM THERMOPHILIC MICROORGANISMS [J].
HENGARTNER, H ;
HARRIS, JI .
FEBS LETTERS, 1975, 55 (01) :282-285
[9]  
Hill AV, 1910, J PHYSIOL-LONDON, V40, piv
[10]   PHOSPHOFRUCTOKINASE - COMPLETE AMINO-ACID-SEQUENCE OF THE ENZYME FROM BACILLUS-STEAROTHERMOPHILUS [J].
KOLB, E ;
HUDSON, PJ ;
HARRIS, JI .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1980, 108 (02) :587-597