SCALE-UP OF RECOMBINANT PROTEIN-PURIFICATION BY HYDROPHOBIC INTERACTION CHROMATOGRAPHY

被引：10
|
作者：
VORAUER, K [1 ]
SKIAS, M [1 ]
TRKOLA, A [1 ]
SCHULZ, P [1 ]
JUNGBAUER, A [1 ]
机构：
[1] UNIV WIEN,INST ANGEW MIKROBIOL,NUSSDORFERLANDE 11,A-1190 VIENNA,AUSTRIA
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 625卷 / 01期
关键词：
D O I：
10.1016/0021-9673(92)87218-W
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The scale-up of hydrophobic interaction chromatography is described. Human recombinant superoxide dismutase was used as a model. The scale-up was performed by keeping the height to diameter (H/D) ratio of the column constant. The success of scale-up was evaluated by reversed-phase high-performance liquid chromatography of the eluted material. The wrong H/D ratio causes decreased resolution.
引用
收藏
页码：33 / 39
页数：7
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