COMPARISON OF 4 INSITU HYBRIDIZATION METHODS, BASED ON DIGOXIGENIN-LABELED AND BIOTIN-LABELED PROBES, IN DETECTING HPV DNA IN MALE CONDYLOMATA ACUMINATA

被引：5

作者：

LASSUS, J ^{[1
]}

NIEMI, KM ^{[1
]}

MARJAMAKI, A ^{[1
]}

SYRJANEN, S ^{[1
]}

KARTAMAA, M ^{[1
]}

LEHMUS, A ^{[1
]}

KROHN, K ^{[1
]}

RANKI, A ^{[1
]}

机构：

[1] UNIV HELSINKI,CENT HOSP,DEPT DERMATOL & VENEREAL DIS,SF-00100 HELSINKI 10,FINLAND

来源：

INTERNATIONAL JOURNAL OF STD & AIDS | 1992年 / 3卷 / 03期

关键词：

HUMAN PAPILLOMAVIRUS (HPV); CONDYLOMA ACUMINATUM; INSITU HYBRIDIZATION; DIGOXIGENIN; BIOTIN;

D O I：

10.1177/095646249200300308

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have compared the efficacy of digoxigenin- and biotin-labelled probes in detecting HPV DNA by in situ hybridization on paraffin-embedded tissue sections of 57 male condyloma-suspect genital lesions. Each biopsy was hybridized with at least three of the following four methods: digoxigenin-labelled HPV DNA probes (Dig-HPV), biotinylated HPV-DNA probes (Bio-HPV), and two commercial methods (ViraType in situ and PathoGene), both based on biotinylated DNA probes. The hybridization products were visualized with colourigenic enzyme substrates. In most biopsies, the 4 methods gave equal results although cross-hybridization was most often found with the low-stringency ViraType method. Dig-HPV 6/11 probes gave positive results about twice as often as either of the commercial methods. No such difference, however, was found for HPV 16/18 probes. DNA of any type of HPV 6/11, 16/18 or 31/33/35 or 51 was detected in 28/43 (65%) of lesions showing condyloma acuminatum histology but in none of the 14 biopsies with no histological signs of HPV infection. In HPV-positive condylomata with no cellular atypia. HPV 6/11 was detected in 87% (13/15), and HPV 16/18 in 27% (4/15). In biopsies with cellular atypia, HPV types 6/11 were detected in 62% (8/13), HPV types 16/18 in 46% (6/13), and HPV types 31/33/35 or 51 in 50% (6/12). In about 50% of the biopsies where at least one hybridization method gave a positive result, either one of the commercial methods gave a negative result. Thus, in situ hybridization using digoxigenin-labelled HPV DNA probes is at least as sensitive a method for HPV detection in paraffin embedded biopsy tissue as in situ hybridization using biotin-labelled probes. Combination of in situ hybridization using digoxigenin-labelled HPV DNA probes with in situ hybridization with biotinylated HPV DNA probes may further improve sensitivity of HPV DNA detection in paraffin-embedded biopsy material.

引用

页码：196 / 203

页数：8

共 23 条

[1]

Boshart M., Gissman L., Ikenberg H., Kleinhein A., Scheurien W., Zur Hausen H., A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical, EMBO J, 3, pp. 1151-1157, (1984)

[2]

Beaudenon S., Kremsdort D., Croissant Q., A novel type of human papillomavirus associated with genital neoplasias, Nature, 321, pp. 246-249, (1986)

[3]

Lorincz A.T., Lancaster W.D., Temple G.F., Cloning and characterization of the DNA of a new human papillomavirus from a woman with dysplasia of the uterine cervix, J Virol, 58, pp. 225-226, (1986)

[4]

Syrjanen K., Parkkinen R., Mantyjarvi R., Human papillomavirus (HPV) type as an important determinant of the natural history of HPV infections in the uterine cervix, Eur J Epidemiol, 1, pp. 180-187, (1985)

[5]

Ikenberg H., Gissman L., Gross G., Grussondorf E., Zur Hausen H., Human papilloma virus type 16 related DNA in genital Bowen's disease and bowenoid papulosis, Int J Cancer, 32, pp. 563-565, (1983)

[6]

Rakoczy P., Sterrett G., Kulski J., Time trends in the prevalence of human papillomavirus infections in archival papanicolaou smears: Analysis by cytology, DNA hybridization, and polymerase chain reaction, J Med Virol, 32, pp. 10-17, (1990)

[7]

Champion M., McCance D., Progressive potential of mild cervical atypia: Prospective cyrological, colposcopic and virological study, Lancet, 2, pp. 237-240, (1986)

[8]

Beckmann A.M., Myerson D., Daling J., Kiviat N., Fenoglio C., McDougall J., Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes, J Med Virol, 16, pp. 265-273, (1985)

[9]

Crum C., Nagal N., Levine R., Silverstein S., In situ hybridization analysis of HPV 16 DNA sequences in early cervical neoplasia, Am J Pathol, 123, pp. 174-182, (1986)

[10]

Burns J., Graham A., Frak C., Fleming F., Evans M., McGee J., Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridization, J Clin Pathol, 40, pp. 858-864, (1987)

← 1 2 3 →