A STRATEGY FOR PHYSICAL MAPPING OF THE HUMAN GENOME

We propose the physical mapping of the human genome from a large genomic library through the use of an ordered set of overlapping DNA clones that span the entire genome. The overlap relations among clones can be determined by repeated screening of the library with probes consisting of pooled cloned inserts. The minimum number of probings necessary for definition of a comprehensive set of overlapping clones can be determined by a simple algorithm. For this approach, most methods are standard in the technique of "chromosome walking." However, suitable instrumentation was required for the repeated and reproducible plating of highly dense large libraries needed in this approach.