APPLICABILITY OF FIXED BRAIN SLICE FOR ULTRASTRUCTURAL-STUDY

被引：9

作者：

LIN, CS

机构：

[1] Department of Physiology and Biophysics, Hahnemann University, Philadelphia

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1990年 / 32卷 / 02期

基金：

美国国家科学基金会;

关键词：

Brain slice; Cortical neuron; Development; Fluorescent dyes; HRP; Lateral geniculate nucleus; Synaptology;

D O I：

10.1016/0165-0270(90)90167-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method is described to study the dendritic morphology and axonal collaterals from fixed slice preparations. Either rhodamine or fluorescein conjugated horseradish peroxidase was used to fill single projecting neurons intracellularly. The identification of projecting neurons was labelled first with rhodamine-coated latex microspheres. After the diaminobenzidine reaction, the filling of detailed morphological characteristics can be examined at both the light and electron microscopic levels. The advantages of this technique are discussed. © 1990.

引用

页码：117 / 121

页数：5

共 11 条

[1] INTRACELLULAR LUCIFER YELLOW INJECTION IN FIXED BRAIN-SLICES COMBINED WITH RETROGRADE TRACING, LIGHT AND ELECTRON-MICROSCOPY [J].