DEVELOPMENT OF A SOLID-PHASE ENZYME-IMMUNOASSAY FOR THE DETECTION OF ANTI-RO AUTOANTIBODIES

被引：0
作者：
DORNER, T
HIEPE, F
MIELKE, F
KIESSIG, ST
LUKOWSKY, A
APOSTOLOFF, E
机构：
[1] HUMBOLDT UNIV,MED SCH CHARITE,DEPT INTERNAL MED,SCHUMANNSTR 20-21,O-1040 BERLIN,GERMANY
[2] HUMBOLDT UNIV,MED SCH CHARITE,INST MED IMMUNOL,O-1086 BERLIN,GERMANY
来源：
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY | 1991年 / 20卷 / 04期
关键词：
ANTI-RO-AUTOANTIBODIES; SYSTEMIC LUPUS ERYTHEMATOSUS; ENZYME IMMUNOASSAY; RO-ANTIGEN PURIFICATION; FAST PROTEIN LIQUID CHROMATOGRAPHY;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
An enzyme immunoassay was developed to detect anti-Ro(SS-A) autoantibodies. Both Ro-antigen components (52 and 60 kD) were purified from a pig spleen extract, using fast protein liquid chromatography (FPLC). Anti-La, anti-RNP, anti-DNA and anti-Sm antibodies do not react to the purified antigen. There was a strong correlation between anti-Ro activity in EIA and the titers in counter immunoelectrophoresis (r(s) = 0,893). Anti-Ro antibodies were found in 54 (69.2%) of 78 SLE sera by the developed EIA.
引用
收藏
页码：267 / 273
页数：7
相关论文
共 50 条
[41]   AN ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF ANTI-IGA ANTIBODIES USING POLYCLONAL HUMAN-IGA [J].
KOSKINEN, S ;
HIRVONEN, M ;
TOLO, H .
JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 179 (01) :51-58
[42]   SOLID-PHASE CHEMILUMINESCENCE IMMUNOASSAY OF METHYLTESTOSTERONE WITH DIRECT READING OF THE MICROTITRE PLATE [J].
VANLOOK, LJ ;
VANPETEGHEM, CH .
ANALYTICA CHIMICA ACTA, 1991, 248 (01) :207-211
[43]   ENZYME-IMMUNOASSAY TECHNIQUES FOR THE DETECTION OF ATRAZINE IN WATER SAMPLES - EVALUATION OF A COMMERCIAL TUBE BASED ASSAY [J].
SHERRY, JP ;
BORGMANN, A .
CHEMOSPHERE, 1993, 26 (12) :2173-2184
[44]   ENZYME-IMMUNOASSAY DETECTION OF ANTIBODIES IN CANINE BLASTOMYCOSIS USING BLASTOMYCES-DERMATITIDIS LYSATE ANTIGENS [J].
SEAWELL, BW ;
LEGENDRE, AM ;
SCALARONE, GM .
MYCOSES, 1990, 33 (9-10) :483-489
[45]   EVALUATION OF AN ENZYME-IMMUNOASSAY (CHLAMYDIAZYME) WITH CONFIRMATORY TEST FOR THE DETECTION OF CHLAMYDIAL ANTIGEN IN URINE FROM MEN [J].
MUMTAZ, G ;
RIDGWAY, GL ;
CLARK, S ;
ALLASONJONES, E .
INTERNATIONAL JOURNAL OF STD & AIDS, 1991, 2 (05) :359-361
[46]   A SENSITIVE ENZYME-IMMUNOASSAY FOR THE DETECTION OF A SYNTHETIC AFFINITY LIGAND, THE REACTIVE YELLOW-13 DYE [J].
REGNAULT, V ;
VALLAR, L ;
RIVAT, C ;
STOLTZ, JF ;
BOSCHETTI, E .
JOURNAL OF IMMUNOASSAY, 1992, 13 (04) :509-520
[47]   ENZYME-IMMUNOASSAY FOR THE DETECTION OF PNEUMOCOCCAL-C POLYSACCHARIDE IN SPUTUM OF PATIENTS WITH PRESUMPTIVE PNEUMOCOCCAL PNEUMONIA [J].
PARKINSON, AJ ;
DAVIDSON, M ;
CAMPBELL, J ;
RABIEGO, ME ;
JOHNSON, C .
SERODIAGNOSIS AND IMMUNOTHERAPY IN INFECTIOUS DISEASE, 1990, 4 (06) :495-503
[48]   ENZYME-IMMUNOASSAY FOR THE DETECTION OF ANTIBODY TO HEPATITIS-E VIRUS-BASED ON SYNTHETIC PEPTIDES [J].
FAVOROV, MO ;
KHUDYAKOV, YE ;
FIELDS, HA ;
KHUDYAKOVA, NS ;
PADHYE, N ;
ALTER, MJ ;
MAST, E ;
POLISH, L ;
YASHINA, TL ;
YARASHEVA, DM ;
ONISCHENKO, GG ;
MARGOLIS, HS .
JOURNAL OF VIROLOGICAL METHODS, 1994, 46 (02) :237-250
[49]   DETECTION OF ANTIBODY TO HEPATITIS-C VIRUS BY 2ND-GENERATION ENZYME-IMMUNOASSAY [J].
CHAUDHARY, RK ;
MACLEAN, C .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1993, 99 (06) :702-704
[50]   RECOMBINANT TECHNOLOGY IN THE PREPARATION OF IMMUNOGEN AND ENZYMATIC TRACER - APPLICATION TO THE DEVELOPMENT OF AN ENZYME-IMMUNOASSAY FOR RAT PROLACTIN [J].
EZAN, E ;
DUCANCEL, F ;
GILLET, D ;
DREVET, P ;
MENEZ, A ;
GROGNET, JM ;
BOULAIN, JC .
JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 169 (02) :205-211
12345