DEVELOPMENT OF A SOLID-PHASE ENZYME-IMMUNOASSAY FOR THE DETECTION OF ANTI-RO AUTOANTIBODIES

被引：0
|
作者：
DORNER, T
HIEPE, F
MIELKE, F
KIESSIG, ST
LUKOWSKY, A
APOSTOLOFF, E
机构：
[1] HUMBOLDT UNIV,MED SCH CHARITE,DEPT INTERNAL MED,SCHUMANNSTR 20-21,O-1040 BERLIN,GERMANY
[2] HUMBOLDT UNIV,MED SCH CHARITE,INST MED IMMUNOL,O-1086 BERLIN,GERMANY
来源：
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY | 1991年 / 20卷 / 04期
关键词：
ANTI-RO-AUTOANTIBODIES; SYSTEMIC LUPUS ERYTHEMATOSUS; ENZYME IMMUNOASSAY; RO-ANTIGEN PURIFICATION; FAST PROTEIN LIQUID CHROMATOGRAPHY;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
An enzyme immunoassay was developed to detect anti-Ro(SS-A) autoantibodies. Both Ro-antigen components (52 and 60 kD) were purified from a pig spleen extract, using fast protein liquid chromatography (FPLC). Anti-La, anti-RNP, anti-DNA and anti-Sm antibodies do not react to the purified antigen. There was a strong correlation between anti-Ro activity in EIA and the titers in counter immunoelectrophoresis (r(s) = 0,893). Anti-Ro antibodies were found in 54 (69.2%) of 78 SLE sera by the developed EIA.
引用
收藏
页码：267 / 273
页数：7
相关论文
共 50 条
  • [1] EVALUATION OF A SOLID-PHASE CELLULAR ENZYME-IMMUNOASSAY FOR DETECTION OF THE SEROLOGICALLY DEFINED MALE ANTIGEN
    REILLY, BD
    GOLDBERG, EH
    JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 142 (01) : 121 - 126
  • [2] QUANTITATION OF ERYTHROCYTE-BOUND IGG BY COMPETITIVE SOLID-PHASE ENZYME-IMMUNOASSAY
    ADACHI, K
    HORII, Y
    NAGATOMO, H
    SHIMIZU, T
    MAKIMURA, S
    JOURNAL OF VETERINARY MEDICAL SCIENCE, 1994, 56 (03) : 499 - 502
  • [3] A SOLID-PHASE ENZYME-IMMUNOASSAY FOR SERUM AMYLOID-A (SAA) PROTEIN - CLINICAL-EVALUATION
    TAKTAK, YS
    LEE, M
    JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 136 (01) : 11 - 16
  • [4] DETERMINATION OF HUMAN-IMMUNOGLOBULIN-A AND SECRETORY IMMUNOGLOBULIN-A IN BRONCHOALVEOLAR LAVAGE FLUIDS BY SOLID-PHASE ENZYME-IMMUNOASSAY
    SATO, T
    KITAJIMA, A
    OHMOTO, S
    CHIKUMA, M
    KADO, M
    NAGAI, S
    IZUMI, T
    TAKEYAMA, M
    MASADA, M
    CLINICA CHIMICA ACTA, 1993, 220 (02) : 145 - 156
  • [5] DEVELOPMENT AND APPLICATION OF AN ENZYME-IMMUNOASSAY FOR TENASCIN
    WASHIZU, K
    KIMURA, S
    HIRAIWA, H
    MATSUNAGA, K
    KUWABARA, M
    ARIYOSHI, Y
    KATO, K
    TAKEUCHI, K
    CLINICA CHIMICA ACTA, 1993, 219 (1-2) : 15 - 22
  • [6] DEVELOPMENT OF AN ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF METAZACHLOR
    SCHOLZ, HM
    HOCK, B
    ANALYTICAL LETTERS, 1991, 24 (03) : 413 - 427
  • [7] Detection of human papillomavirus type-16 DNA utilising microtitre-plate based amplification reactions and a solid-phase enzyme-immunoassay detection system
    Cavuslu, S
    Starkey, WG
    Kaye, JN
    Biswas, C
    Mant, C
    Kell, B
    Rice, P
    Best, JM
    Cason, J
    JOURNAL OF VIROLOGICAL METHODS, 1996, 58 (1-2) : 59 - 69
  • [8] Development of a solid-phase cleanup and portable rapid flow-through enzyme immunoassay for the detection of ochratoxin a in roasted coffee
    Sibanda, L
    De Saeger, S
    Barna-Vetro, I
    Van Peteghem, C
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (24) : 6964 - 6967
  • [9] ENZYME-IMMUNOASSAY FOR THE DETECTION OF LEVAMISOLE IN MEAT AND MILK
    SILVERLIGHT, JJ
    JACKMAN, R
    ANALYST, 1994, 119 (12) : 2705 - 2706
  • [10] Role of anti-Ro autoantibodies in systemic lupus erythematosus patients with recurrent myositis
    S Umer
    S Hayat
    G Caldito
    SM Berney
    Arthritis Research & Therapy, 14 (Suppl 3):
12345