BIOSENSOR DEVELOPMENT WITH A GLUTAMATE RECEPTOR ION-CHANNEL RECONSTITUTED IN A LIPID BILAYER

被引：44
|
作者：
UTO, M
MICHAELIS, EK
HU, IF
UMEZAWA, Y
KUWANA, T
机构：
[1] UNIV KANSAS,CTR BIOMED RES,LAWRENCE,KS 66046
[2] UNIV KANSAS,CTR BIOANALYT RES,LAWRENCE,KS 66046
来源：
ANALYTICAL SCIENCES | 1990年 / 6卷 / 02期
关键词：
biosensor; Glutamate receptor ion channel; multi-channel sensor; single-channel sensor;
D O I：
10.2116/analsci.6.221
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A glutamate receptor ion channel (RIC) protein, isolated and purified from rat brains, was reconstituted into artificial bilayer lipid membranes. This RIC protein was found to serve as a recognition site for a sensitive detection of L-glutamate. Two types of RIC sensors were tested. When the RIC was reconstituted as a single protein in a patch-clamp membrane configuration, digital “off/on” signals were obtained for L-glutamate. With multiple proteins as a multi-channel sensor, the integrated dc signals were related to the glutamate concentration. The results from these two configurations are further discussed in terms of signal amplification and concentration dependence of the sensor. © 1990, The Japan Society for Analytical Chemistry. All rights reserved.
引用
收藏
页码：221 / 225
页数：5
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