RECOMBINANT RABBIT HEMORRHAGIC-DISEASE VIRUS CAPSID PROTEIN EXPRESSED IN BACULOVIRUS SELF-ASSEMBLES INTO VIRUS-LIKE PARTICLES AND INDUCES PROTECTION

被引：148
作者：
LAURENT, S
VAUTHEROT, JF
MADELAINE, MF
LEGALL, G
RASSCHAERT, D
机构：
[1] INRA, UNITE VIROL & IMMUNOL MOLEC, F-78350 JOUY EN JOSAS, FRANCE
[2] CTR NATL ETUD VET & ALIMENTAIRES, F-22440 PLOUFRAGAN, FRANCE
来源：
JOURNAL OF VIROLOGY | 1994年 / 68卷 / 10期
关键词：
D O I：
10.1128/JVI.68.10.6794-6798.1994
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
VP60, the unique component of rabbit hemorrhagic disease virus capsid, was expressed in the baculovirus system. The recombinant VP60, released in the supernatant of infected insect cells, assembled without the need of any other viral component to form viruslike particles (VLPs), structurally and immunologically indistinguishable from the rabbit hemorrhagic disease virion. Intramuscular vaccination of rabbits with the VLPs conferred complete protection in 15 days; this protection was found to be effective from the fifth day after VLP injection and was accompanied by a strong humoral response.
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页码：6794 / 6798
页数：5
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