THE ENTRY OF JUNIN VIRUS INTO VERO CELLS

被引：54

作者：

CASTILLA, V ^{[1
]}

MERSICH, SE ^{[1
]}

CANDURRA, NA ^{[1
]}

DAMONTE, EB ^{[1
]}

机构：

[1] UNIV BUENOS AIRES, FAC CIENCIAS EXACTAS & NAT, DEPT QUIM BIOL, VIROL LAB, RA-1428 BUENOS AIRES, ARGENTINA

来源：

ARCHIVES OF VIROLOGY | 1994年 / 136卷 / 3-4期

关键词：

D O I：

10.1007/BF01321064

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect oflysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infection. Virus internalization was inhibited and viral protein expression was not detected. When the extracellular medium was buffered at low pH, the ammonium chloride induced block on JV infection was overcome. Furthermore, JV was able to induce fusion of infected cells at pH 5.5 leading to polykaryoctye formation. Taken together, these results demonstrate that JV entry occurs through an endocytic mechanism requiring a low pH dependent membrane fusion.

引用

页码：363 / 374

页数：12

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