EXPRESSION OF HUMAN RECOMBINANT BETA(2)-GLYCOPROTEIN-I WITH ANTICARDIOLIPIN ANTIBODY COFACTOR ACTIVITY

被引：13
|
作者：
KOUTS, S
BUNN, CL
STEINKASSERER, A
KRILIS, S
机构：
[1] UNIV NEW S WALES, ST GEORGE HOSP, DEPT IMMUNOL ALLERGY & INFECT DIS, KOGARAH, NSW 2217, AUSTRALIA
[2] UNIV NEW S WALES, ST GEORGE HOSP, DEPT MED, KOGARAH, NSW 2217, AUSTRALIA
[3] BIOTECH AUSTRALIA, ROSEVILLE, AUSTRALIA
[4] UNIV OXFORD, DEPT BIOCHEM, MRC, IMMUNOHISTOCHEM UNIT, OXFORD, ENGLAND
来源：
FEBS LETTERS | 1993年 / 326卷 / 1-3期
基金：
英国医学研究理事会;
关键词：
BETA(2)-GLYCOPROTEIN-I; ANTICARDIOLIPIN ANTIBODY; ANTIPHOSPHOLIPID ANTIBODY;
D O I：
10.1016/0014-5793(93)81771-Q
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To enable the synthesis of beta2-glycoprotein I mutants we have established a stable Chinese hamster ovary cell line that expresses human beta2-glycoprotein I up to 2.9 mug/10(6) cells/day. Recombinant beta2-glycoprotein I is identical to the purified native protein with respect to cofactor activity revealed in a modified anti-cardiolipin ELISA. Autoimmune type anti-cardiolipin antibody requires recombinant beta2-glycoprotein I in a dose-dependent manner to bind cardiolipin whilst binding of infectious type antibody is inhibited. The purified recombinant beta2-glycoprotein I in serum free medium exists as two oligosaccharide species which upon deglycosylation have identical apparent molecular weight to the deglycosylated native protein.
引用
收藏
页码：105 / 108
页数：4
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