DEVELOPMENT AND CHROMOSOMAL LOCALIZATION OF GENOME-SPECIFIC MARKERS BY POLYMERASE CHAIN-REACTION IN BRASSICA

被引：105

作者：

QUIROS, CF

HU, J

THIS, P

CHEVRE, AM

DELSENY, M

机构：

[1] UNIV PERPIGNAN,PHYSIOL & PLANT MOLEC BIOL LAB,F-66025 PERPIGNAN,FRANCE

[2] INRA,IMPROVEMENT PLANTS STN,F-35650 LE RHEU,FRANCE

来源：

THEORETICAL AND APPLIED GENETICS | 1991年 / 82卷 / 05期

关键词：

CHROMOSOMES MARKERS; RAPD MARKERS; COLE CROPS; RAPESEED; GENE MAPPING;

D O I：

10.1007/BF00226801

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

This paper reports the application of the RAPD (random amplification of polymorphic DNA sequence) markers in Brassica genetics. Forty-seven arbitrary decamer oligonucleotides were used as primers to amplify genomic DNA by polymerase chain reaction. Some of the amplified products were genome specific and could be found in both diploid and derived amphidiploid species. Of a total of 65 such markers, 16 were A genome, 37 B genome, and 12 C genome specific. Of the 37 B-genome-specific markers, 11 were mapped on four independent chromosomes of B. nigra with the aid of existing B. napus-nigra disomic alien addition lines.

引用

页码：627 / 632

页数：6

共 19 条

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[2]

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[3]

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