HUMAN INTERFERON-PRODUCTION WITH DIPLOID FIBROBLAST CELLS GROWN ON MICRO-CARRIERS

被引：36

作者：

GIARD, DJ ^{[1
]}

LOEB, DH ^{[1
]}

THILLY, WG ^{[1
]}

WANG, DIC ^{[1
]}

LEVINE, DW ^{[1
]}

机构：

[1] MIT,DEPT NUTR & FOOD SCI,CAMBRIDGE,MA 02139

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 03期

关键词：

D O I：

10.1002/bit.260210306

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A variety of diploid human fibroblast lines have been successfully grown to high densities (>106 cell/ml) on recently developed microcarriers. Interferon induction using poly I·poly C and a superinduction procedure resulted in yields greater than 10,000 units/ml with one cell line. A direct comparison of microcarrier cultures to roller bottle cultures showed equivalent interferon yields on a per cell basis and some apparent differences relating to optimum inducer concentrations and kinetics of interferon accumulation. Copyright © 1979 John Wiley & Sons, Inc.

引用

页码：433 / 442

页数：10

共 17 条

[1]

AOKI FY, 1975, LANCET, V1, P660

[2] MASS-PRODUCTION OF HUMAN INTERFERON IN DIPLOID CELLS STIMULATED BY POLY-I-C [J].