PHOSPHOLIPASE-D IS PRESENT ON GOLGI-ENRICHED MEMBRANES AND ITS ACTIVATION BY ADP-RIBOSYLATION FACTOR IS SENSITIVE TO BREFELDIN-A

被引：175

作者：

KTISTAKIS, NT ^{[1
]}

BROWN, HA ^{[1
]}

STERNWEIS, PC ^{[1
]}

ROTH, MG ^{[1
]}

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT PHARMACOL,DALLAS,TX 75235

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 11期

关键词：

D O I：

10.1073/pnas.92.11.4952

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

ADP ribosylation factor (ARF) is a small guanosine triphosphate (GTP)-binding protein that regulates the binding of coat proteins to membranes and is required for several stages of vesicular transport, ARF also stimulates phospholipase D (PLD) activity, which can alter the lipid content of membranes by conversion of phospholipids into phosphatidic acid, Abundant PLD activity was found in Golgi-enriched membranes from several cell lines, Golgi PLD activity was greatly stimulated by ARF and GTP analogs and this stimulation could be inhibited by brefeldin A (BFA), a drug that blocks binding of ARF to Golgi membranes, Furthermore, in Golgi membranes from BFA-resistant PtK1 cells, basal PLD activity was high and not stimulated by exogenous ARF or GTP analogs, Thus, ARF activates PLD on the Golgi complex, suggesting a possible link between transport events and the underlying architecture of the lipid bilayer.

引用

页码：4952 / 4956

页数：5

共 33 条

[1] RECONSTITUTION OF THE TRANSPORT OF PROTEIN BETWEEN SUCCESSIVE COMPARTMENTS OF THE GOLGI MEASURED BY THE COUPLED INCORPORATION OF N-ACETYLGLUCOSAMINE [J].

BALCH, WE ;

DUNPHY, WG ;

BRAELL, WA ;

ROTHMAN, JE .

CELL, 1984, 39 (02) :405-416

[2] AN ESSENTIAL ROLE FOR A PHOSPHOLIPID TRANSFER PROTEIN IN YEAST GOLGI FUNCTION [J].

BANKAITIS, VA ;

AITKEN, JR ;

CLEVES, AE ;

DOWHAN, W .

NATURE, 1990, 347 (6293) :561-562

[3] ADP-RIBOSYLATION FACTOR, A SMALL GTP-DEPENDENT REGULATORY PROTEIN, STIMULATES PHOSPHOLIPASE-D ACTIVITY [J].

BROWN, HA ;

GUTOWSKI, S ;

MOOMAW, CR ;

SLAUGHTER, C ;

STERNWEIS, PC .

CELL, 1993, 75 (06) :1137-1144

[4]

BROWN HA, 1995, IN PRESS J BIOL CHEM, V270

[5]

BROWN HA, 1995, IN PRESS METHODS ENZ, V257

[6] PHOSPHOLIPASE-D - A DOWNSTREAM EFFECTOR OF ARF IN GRANULOCYTES [J].

COCKCROFT, S ;

THOMAS, GMH ;

FENSOME, A ;

GENY, B ;

CUNNINGHAM, E ;

GOUT, I ;

HILES, I ;

TOTTY, NF ;

TRUONG, Q ;

HSUAN, JJ .

SCIENCE, 1994, 263 (5146) :523-526

[7]

COMPANS RW, 1994, METHOD CELL BIOL, V43, P3

[8] BREFELDIN-A INHIBITS GOLGI MEMBRANE-CATALYZED EXCHANGE OF GUANINE-NUCLEOTIDE ONTO ARF PROTEIN [J].

DONALDSON, JG ;

FINAZZI, D ;

KLAUSNER, RD .

NATURE, 1992, 360 (6402) :350-352

[9] ADP-RIBOSYLATION FACTOR, A SMALL GTP-BINDING PROTEIN, IS REQUIRED FOR BINDING OF THE COATOMER PROTEIN BETA-COP TO GOLGI MEMBRANES [J].

DONALDSON, JG ;

CASSEL, D ;

KAHN, RA ;

KLAUSNER, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6408-6412

[10] BINDING OF ARF AND BETA-COP TO GOLGI MEMBRANES - POSSIBLE REGULATION BY A TRIMERIC G-PROTEIN [J].

DONALDSON, JG ;

KAHN, RA ;

LIPPINCOTTSCHWARTZ, J ;

KLAUSNER, RD .

SCIENCE, 1991, 254 (5035) :1197-1199

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