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COMPARISON OF IMMUNOHISTOCHEMISTRY, ACID-FAST STAINING, AND CULTIVATION FOR DETECTION OF MYCOBACTERIUM-PARATUBERCULOSIS IN GOATS
被引：30
作者：
THORESEN, OF
FALK, K
EVENSEN, O
机构：
[1] Central Veterinary Laboratory, Oslo
关键词：
D O I：
10.1177/104063879400600210
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Forty-seven paired specimens of ileum and mesenterial lymph nodes from goats originating from 2 herds with paratuberculosis were investigated. Culture of the specimens for Mycobacterium paratuberculosis was compared with Ziehl-Neelsen staining and with immunohistochemical tests on paraffin-embedded tissue sections, using a M. paratuberculosis immune serum and an avidin-biotin-alkaline phosphatase method. Immunohistochemical techniques detected most positive samples and produced more clearly visible reactions than did acid-fast staining. Eighteen of 47 samples were positive by immunohistochemical methods, whereas 15 and 10 were positive for acid-fast staining and culture, respectively. Immunohistochemical techniques may represent a valuable adjunct to standard techniques for diagnosis of paratuberculosis in goats.
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页码：195 / 199
页数：5
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