COMPARISON OF IMMUNOHISTOCHEMISTRY, ACID-FAST STAINING, AND CULTIVATION FOR DETECTION OF MYCOBACTERIUM-PARATUBERCULOSIS IN GOATS

被引:30
作者
THORESEN, OF
FALK, K
EVENSEN, O
机构
[1] Central Veterinary Laboratory, Oslo
关键词
D O I
10.1177/104063879400600210
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Forty-seven paired specimens of ileum and mesenterial lymph nodes from goats originating from 2 herds with paratuberculosis were investigated. Culture of the specimens for Mycobacterium paratuberculosis was compared with Ziehl-Neelsen staining and with immunohistochemical tests on paraffin-embedded tissue sections, using a M. paratuberculosis immune serum and an avidin-biotin-alkaline phosphatase method. Immunohistochemical techniques detected most positive samples and produced more clearly visible reactions than did acid-fast staining. Eighteen of 47 samples were positive by immunohistochemical methods, whereas 15 and 10 were positive for acid-fast staining and culture, respectively. Immunohistochemical techniques may represent a valuable adjunct to standard techniques for diagnosis of paratuberculosis in goats.
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页码:195 / 199
页数:5
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