P53 DOMAINS - IDENTIFICATION AND CHARACTERIZATION OF 2 AUTONOMOUS DNA-BINDING REGIONS

被引：213

作者：

WANG, Y ^{[1
]}

REED, M ^{[1
]}

WANG, P ^{[1
]}

STENGER, JE ^{[1
]}

MAYR, G ^{[1
]}

ANDERSON, ME ^{[1
]}

SCHWEDES, JF ^{[1
]}

TEGTMEYER, P ^{[1
]}

机构：

[1] SUNY STONY BROOK,DEPT MICROBIOL,STONY BROOK,NY 11794

来源：

GENES & DEVELOPMENT | 1993年 / 7卷 / 12B期

关键词：

P53; DOMAINS; TETRAMERS; DNA BINDING; TRANSACTIVATION;

D O I：

10.1101/gad.7.12b.2575

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have investigated the DNA-binding, oligomerization, and trans-activation functions of isolated segments of murine p53. We find that p53 has two autonomous DNA-binding regions. One domain, from amino acid 280 to 390, forms stable tetramers and binds DNA nonspecifically. The biological significance, if any, of this DNA-binding activity is not known. A second domain, from amino acid 80 to 290 does not form stable tetramers under stringent conditions but binds DNA both specifically and nonspecifically. The specific DNA-binding function of p53, therefore, resides in the highly conserved central region of the protein and does not require stable tetramerization. Amino acids 1-290, which include both the specific DNA-binding domain and the amino-terminal acidic region, activate a p53-specific promoter in vivo. This finding strongly argues that the DNA-binding activity of p53 segment 80-290 is physiologically significant. The role of tetramerization in p53 function remains to be determined.

引用

页码：2575 / 2586

页数：12

共 66 条

[1] OVERLAP OF THE P53-RESPONSIVE ELEMENT AND CAMP-RESPONSIVE ELEMENT IN THE ENHANCER OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I [J].