BACULOVIRUS-EXPRESSED HERPES-SIMPLEX VIRUS TYPE-2 GLYCOPROTEIN-D IS IMMUNOGENIC AND PROTECTIVE AGAINST LETHAL HSV CHALLENGE

被引：24

作者：

LANDOLFI, V ^{[1
]}

ZARLEY, CD ^{[1
]}

ABRAMOVITZ, AS ^{[1
]}

FIGUEROA, N ^{[1
]}

WU, SL ^{[1
]}

BLASIAK, M ^{[1
]}

ISHIZAKA, ST ^{[1
]}

MISHKIN, EM ^{[1
]}

机构：

[1] LEDERLE PRAXIS BIOL, DEPT VIRAL VACCINE RES & DEV, PEARL RIVER, NY 10965 USA

来源：

VACCINE | 1993年 / 11卷 / 04期

关键词：

HERPES SIMPLEX VIRUS; GLYCOPROTEIN-D; BACULOVIRUS EXPRESSION; IMMUNOGENICITY; PROTECTIVITY;

D O I：

10.1016/0264-410X(93)90280-B

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Herpes simplex virus type 2 glycoprotein D (gD2) was cloned and expressed in the baculovirus-Spodoptera frugiperda system. Milligram quantities of glycoprotein were recovered from suspension culture and subjected to purification by ion-exchange and immunoaffinity chromatography. The resultant purified gD existed as a homogeneous 57 500 M(W) monomeric species demonstrating reactivity with anti-gD monoclonal antibodies including those directed at a non-sequential neutralizing epitope of gD. Immunization of Balb/c mice with doses of 0.1-10.0 mug of AlPO4-absorbed gD resulted in elicitation of humoral and cellular responses to both HSV1 and HSV2 as well as to purified gD1 and gD2. Immunized mice receiving an infectious dose of 1 x 10(6) p.f.u. of HSV2 via the footpad route were significantly protected against infection at all doses tested when compared with unimmunized AlPO4 and uninoculated control animals.

引用

页码：407 / 414

页数：8

共 44 条

[1] SPECIFICITY OF THE IMMUNE-RESPONSE OF MICE TO HERPES-SIMPLEX VIRUS GLYCOPROTEINS-B AND GLYCOPROTEINS-D CONSTITUTIVELY EXPRESSED ON L-CELL LINES
BLACKLAWS, BA
NASH, AA
DARBY, G
[J]. JOURNAL OF GENERAL VIROLOGY, 1987, 68 : 1103 - 1114
[2] A HERPESVIRUS ANTIGEN IN HUMAN PREMALIGNANT AND MALIGNANT CERVICAL BIOPSIES AND EXPLANTS
CABRAL, GA
FRY, D
MARCIANOCABRAL, F
LUMPKIN, C
MERCER, L
GOPLERUD, D
[J]. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1983, 145 (01) : 79 - 86
[3] CLINICAL EFFICACY OF A HERPES-SIMPLEX SUBUNIT VACCINE
CAPPEL, R
SPRECHER, S
DECUYPER, F
DEBRAEKELEER, J
[J]. JOURNAL OF MEDICAL VIROLOGY, 1985, 16 (02) : 137 - 145
[4] TYPE-COMMON CP-1 ANTIGEN OF HERPES-SIMPLEX VIRUS IS ASSOCIATED WITH A 59,000-MOLECULAR-WEIGHT ENVELOPE GLYCOPROTEIN
COHEN, GH
KATZE, M
HYDREANSTERN, C
EISENBERG, RJ
[J]. JOURNAL OF VIROLOGY, 1978, 27 (01) : 172 - 181
[5] NUCLEOTIDE-SEQUENCE OF BOVINE ROTAVIRUS GENE-1 AND EXPRESSION OF THE GENE-PRODUCT IN BACULOVIRUS
COHEN, J
CHARPILIENNE, A
CHILMONCZYK, S
ESTES, MK
[J]. VIROLOGY, 1989, 171 (01) : 131 - 140
[6] LOCALIZATION OF EPITOPES OF HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEIN-D
EISENBERG, RJ
LONG, D
DELEON, MP
MATTHEWS, JT
SPEAR, PG
GIBSON, MG
LASKY, LA
BERMAN, P
GOLUB, E
COHEN, GH
[J]. JOURNAL OF VIROLOGY, 1985, 53 (02) : 634 - 644
[7] SYNTHESIS AND IMMUNOGENICITY OF THE ROTAVIRUS MAJOR CAPSID ANTIGEN USING A BACULOVIRUS EXPRESSION SYSTEM
ESTES, MK
CRAWFORD, SE
PENARANDA, ME
PETRIE, BL
BURNS, JW
CHAN, WK
ERICSON, B
SMITH, GE
SUMMERS, MD
[J]. JOURNAL OF VIROLOGY, 1987, 61 (05) : 1488 - 1494
[8] FRENKEL LM, 1990, 30TH ICAAC M
[9] ANTI-GLYCOPROTEIN-D ANTIBODIES THAT PERMIT ADSORPTION BUT BLOCK INFECTION BY HERPES-SIMPLEX VIRUS-1 PREVENT VIRION CELL-FUSION AT THE CELL-SURFACE
FULLER, AO
SPEAR, PG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (15) : 5454 - 5458
[10] HEBERKATZ E, 1985, J IMMUNOL, V135, P1385

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