DETERMINATION OF PREDNISOLONE AND PREDNISONE IN PLASMA BY LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

被引：12

作者：

YAMAGUCHI, M

ISHIDA, J

YOSHITAKE, T

NAKAMURA, M

机构：

[1] Faculty of Pharmaceutical Sciences, Fukuoka University, Johnan-ku, Fukuoka, 814-01, Nanakuma

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 242卷 / 01期

关键词：

LIQUID CHROMATOGRAPHY; PREDNISOLONE; PREDNISONE; PLASMA;

D O I：

10.1016/0003-2670(91)87054-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

After a single-step extraction from plasma (250-mu-l) with dichloromethane, the drugs and dexamethasone (internal standard) are oxidized by copper(II) acetate to the corresponding glyoxal and converted into the fluorescent quinoxalines by reaction with 1,2-diamino-4,5-methylenedioxybenzene. The quinoxalines are separated within 55 min by reversed-phase liquid chromatography with isocratic elution. The detection limits for prednisolone and prednisone added to plasma are 3 ng ml-1 in plasma (signal-to-noise ratio = 3).

引用

页码：113 / 116

页数：4

共 10 条

[1]

CHENG MH, 1988, CLIN CHEM, V34, P1897

[2]

FREY FJ, 1979, CLIN CHEM, V25, P1944

[3] 1,2-DIAMINO-4,5-METHYLENEDIOXYBENZENE AS A HIGHLY SENSITIVE FLUOROGENIC REAGENT FOR ALPHA-DICARBONYL COMPOUNDS [J].