AN IMPROVED UREA SPECIFIC ENZYME ELECTRODE

被引：66

作者：

GUILBAULT, GG

MONTALVO, JG

机构：

[1] Chemistry Department, Louisiana State University in New Orleans, New Orleans

来源：

ANALYTICAL LETTERS | 1969年 / 2卷 / 05期

关键词：

acrylamide polymer; Beckman cation electrode; cellophane; nylon netting; urea; urease;

D O I：

10.1080/00032716908051300

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The enzyme urease was immobilized in a layer of acrylamide polymer on the surface of a Beckman cationic electrode sensitive to ammonium ion. The substrate urea diffuses to the enzyme electrode and reacts with the immobilized enzyme to produce ammonium ion at the surface of the glass electrode. By placing a thin film of cellophane around the enzyme gel layer to prevent leaching of urease into the surrounding solution, an electrode could be used continuously for over 21 days at 25[ddot]C with no loss of activity. © 1969, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：283 / +

页数：1

共 9 条

[1] PREPARATION OF IMMOBILIZED CHOLINESTERASE FOR USE IN ANALYTICAL CHEMISTRY [J].