DISRUPTION OF PACHYTENE DNA METABOLISM IN MALE-MICE WITH CHROMOSOMALLY-DERIVED STERILITY

被引：20

作者：

HOTTA, Y

CHANDLEY, AC

STERN, H

SEARLE, AG

BEECHEY, CV

机构：

[1] WESTERN GEN HOSP,MRC,CLIN & POPULAT CYTOGENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND

[2] MRC,RADIOBIOL UNIT,HARWELL OX11 ORD,ENGLAND

来源：

CHROMOSOMA | 1979年 / 73卷 / 03期

关键词：

D O I：

10.1007/BF00288693

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA metabolism was analyzed in spermatocytes of mice that were sterile either because of X-autosome or autosome-autosome translocations, or because of trisomy. In the strains analyzed, spermatogenic development is arrested by metaphase I or soon thereafter. In all such strains, a disruption of the normal pattern of pachytene DNA metabolism occurred. Prepachytene metabolism appeared normal. Disruption was manifest in both the level of endogenously generated nicks during pachytene and in the distribution of nicks among the different DNA sequence classes. Nicking was more intense in the steriles and tended to be randomized in distribution. Satellite DNA underwent pachytene nick-repair in the steriles but not in fertile controls. The repair capacity of spermatocytes from steriles was equal to that of the fertiles; the higher frequency of nicks in the steriles was due to a persistence of nicking activity. © 1979 Springer-Verlag.

引用

页码：287 / 300

页数：14

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