FUNCTIONAL-ANALYSIS OF THE TRANSCRIPTIONAL PROMOTER FOR THE CYP1A1 GENE

被引:56
作者
JONES, KW [1 ]
WHITLOCK, JP [1 ]
机构
[1] STANFORD UNIV,MED CTR,SCH MED,DEPT PHARMACOL,STANFORD,CA 94305
关键词
D O I
10.1128/MCB.10.10.5098
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In mouse hepatoma cells, the environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) increases the transcription rate of the CYP1A1 gene, which encodes a cytochrome P-450 enzyme. In this study, we analyzed the DNA region immediately upstream of the CYP1A1 gene. A domain that extends upstream to nucleotide -166 was found to function as a transcriptional promoter. The promoter was silent when uncoupled from the dioxin-responsive enhancer located farther upstream. DNase footprinting experiments indicated that nuclear proteins interact with distinct domains of the promoter in a TCDD-independent fashion. Mutational analyses indicated that the CYP1A1 promoter contains at least three functional domains, including a TATAAA sequence, a CCAAT box transcription factor/nuclear factor I-like recognition motif, and a guanine-rich G box.
引用
收藏
页码:5098 / 5105
页数:8
相关论文
共 58 条
[41]  
MILLER AG, 1983, J BIOL CHEM, V258, P3523
[42]   TRANSCRIPTIONAL REGULATION IN MAMMALIAN-CELLS BY SEQUENCE-SPECIFIC DNA-BINDING PROTEINS [J].
MITCHELL, PJ ;
TJIAN, R .
SCIENCE, 1989, 245 (4916) :371-378
[43]   FACTORS INVOLVED IN SPECIFIC TRANSCRIPTION BY MAMMALIAN RNA POLYMERASE-II - PURIFICATION, GENETIC SPECIFICITY, AND TATA BOX-PROMOTER INTERACTIONS OF TFIID [J].
NAKAJIMA, N ;
HORIKOSHI, M ;
ROEDER, RG .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (10) :4028-4040
[44]   REGULATION OF MOUSE CYP1A1 GENE-EXPRESSION BY DIOXIN - REQUIREMENT OF 2 CIS-ACTING ELEMENTS DURING INDUCTION [J].
NEUHOLD, LA ;
SHIRAYOSHI, Y ;
OZATO, K ;
JONES, JE ;
NEBERT, DW .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (06) :2378-2386
[45]   DIOXIN-INDUCIBLE ENHANCER REGION UPSTREAM FROM THE MOUSE P1450 GENE AND INTERACTION WITH A HETEROLOGOUS SV40 PROMOTER [J].
NEUHOLD, LA ;
GONZALEZ, FJ ;
JAISWAL, AK ;
NEBERT, DW .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1986, 5 (05) :403-411
[46]   SEQUENCE-SPECIFIC INTERACTIONS OF NUCLEAR FACTORS WITH THE INSULIN GENE ENHANCER [J].
OHLSSON, H ;
EDLUND, T .
CELL, 1986, 45 (01) :35-44
[47]   2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN AND RELATED HALOGENATED AROMATIC-HYDROCARBONS - EXAMINATION OF THE MECHANISM OF TOXICITY [J].
POLAND, A ;
KNUTSON, JC .
ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1982, 22 :517-554
[48]  
ROSENFELD PJ, 1986, J BIOL CHEM, V261, P1398
[50]   PROMOTER SPECIFICITY AND MODULATION OF RNA POLYMERASE-II TRANSCRIPTION [J].
SALTZMAN, AG ;
WEINMANN, R .
FASEB JOURNAL, 1989, 3 (06) :1723-1733