FUNCTIONAL-ANALYSIS OF THE TRANSCRIPTIONAL PROMOTER FOR THE CYP1A1 GENE

被引：56

作者：

JONES, KW ^{[1
]}

WHITLOCK, JP ^{[1
]}

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT PHARMACOL,STANFORD,CA 94305

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 10期

关键词：

D O I：

10.1128/MCB.10.10.5098

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In mouse hepatoma cells, the environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) increases the transcription rate of the CYP1A1 gene, which encodes a cytochrome P-450 enzyme. In this study, we analyzed the DNA region immediately upstream of the CYP1A1 gene. A domain that extends upstream to nucleotide -166 was found to function as a transcriptional promoter. The promoter was silent when uncoupled from the dioxin-responsive enhancer located farther upstream. DNase footprinting experiments indicated that nuclear proteins interact with distinct domains of the promoter in a TCDD-independent fashion. Mutational analyses indicated that the CYP1A1 promoter contains at least three functional domains, including a TATAAA sequence, a CCAAT box transcription factor/nuclear factor I-like recognition motif, and a guanine-rich G box.

引用

页码：5098 / 5105

页数：8

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