ISOLATION AND CHARACTERIZATION OF A TOXIC PHOSPHOLIPASE-A2 IN THE SPITTING COBRA (NAJA-MOSSAMBICA-MOSSAMBICA) VENOM

被引：17
作者：
MARTINMOUTOT, N
ROCHAT, H
机构：
[1] INSERM, Groupe U.172, Chimie et mode d'action des toxines animales, Laboratoire de Biochimie, Faculté de Médecine, Secteur Nord, Boulevard Pierre Dramard, 13326-Marseille
来源：
TOXICON | 1979年 / 17卷 / 02期
关键词：
D O I：
10.1016/0041-0101(79)90291-5
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
N. Martin-Moutot and H. Rochat. Isolation and characterization of a toxic phospholipase A2 in the spitting cobra (Naja mossambica mossambica) venom. Toxicon 17, 127-136, 1979.-A phospholipase A2 from the spitting cobra (Naja mossambica mossambica) was purified by chromatographic procedures involving gel filtration on Sephadex G-50 and ion exchange on Amberlite IRC-50. The purified enzyme was homogeneous according to physico-chemical criteria as well as chemical purity tests. This protein was characterized by amino acid composition, determination of N- and C- terminal amino acid sequences, isoelectric pH, molecular weight. It exhibits phospholipase activity and toxicity to mice. Positional specificity towards phosphatidylcholine showed that it belongs to the A2 type. Optimal activity was observed at an alkaline pH and calcium was required for phospholipase activity. © 1979.
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页码：127 / 136
页数：10
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