Production and characterization of xanthan gum by different strains of Xanthomonas campestris isolated from various natural sources in submerged culture

被引:0
作者
Alvarado Almarza, Carlos [1 ]
Romero Romero, Fredy [2 ]
机构
[1] Univ Carabobo, Chem Res Ctr, CIQ UC, Valencia, Spain
[2] Simon Rodriguez Expt Natl Univ, Postgrad Decanate, Reg Nucleus Postgrad Valencia, Valencia, Spain
来源
INGENIERIA UC | 2013年 / 20卷 / 01期
关键词
Exopolysaccharide; Phytopathogenic bacteria; Xanthomonas campestris; Xanthan gum;
D O I
暂无
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
The xanthan gum is an exopolysaccharide (EPS) of microbial origin, produced by the bacteria of the X. campestris. The objective of this research was to evaluate production of xanthan gum by different strains of X. campestris isolated from various natural sources in submerge culture. Four bacterial strains of X. campestris were isolated from American and Romaine lettuce (Lactuca sativa) and broccoli (Brassica oleacea var. italic). Kinetic parameters of growth were determined in discontinuous cultivation of the isolated strains and one of reference. The effect of the oxygen and calcium in the cultivation media was evaluated about the growth of the reference strain demonstrating their influence in the growth of the microorganism and in the production of the exopolysaccharide. Kinetic parameters of studied strains showed appropriate tendencies. Xanthan gum was obtained from LR1-UCIN strain with yield of 8.84 g/L, viscosity of 1814 cP, and content of remains of piruvato of 5,10 %.
引用
收藏
页码:34 / 41
页数:8
相关论文
共 20 条
[1]  
Azuaje R., 1999, VENEZUELAN SCIENTIST, V50, P201
[2]   Xanthan gum production under several operational conditions:: molecular structure and rheological properties [J].
Casas, JA ;
Santos, VE ;
García-Ochoa, F .
ENZYME AND MICROBIAL TECHNOLOGY, 2000, 26 (2-4) :282-291
[3]  
Flickinger M., 1999, ENCY BIOPROCESS TECH, P2695
[4]   Characterization of xanthan gum biosynthesis in a centrifugal, packed-bed reactor using metabolic flux analysis [J].
Hsu, CH ;
Lo, YM .
PROCESS BIOCHEMISTRY, 2003, 38 (11) :1617-1625
[5]   Optimization of xanthan gum production by Xanthomonas campestris grown in molasses [J].
Kalogiannis, S ;
Iakovidou, G ;
Liakopoulou-Kyriakides, M ;
Kyriakidis, DA ;
Skaracis, GN .
PROCESS BIOCHEMISTRY, 2003, 39 (02) :249-256
[6]   Xanthomonas campestris pv. campestris gum mutants:: Effects on xanthan biosynthesis and plant virulence [J].
Katzen, F ;
Ferreiro, DU ;
Oddo, CG ;
Ielmini, MV ;
Becker, A ;
Pühler, A ;
Ielpi, L .
JOURNAL OF BACTERIOLOGY, 1998, 180 (07) :1607-1617
[7]  
Lide D. R., 2005, CRC HDB THERMOPHYSIC, DOI DOI 10.1201/9781003067719
[8]  
Mata J., 2006, THESIS
[9]   Effect of virulence and serial transfers of Xanthomonas campestris on xanthan gum production [J].
Nitschke, M ;
Rodrigues, V .
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2000, 31 (01) :58-60
[10]   Xanthan production by Xanthomonas campestris in batch cultures [J].
Papagianni, M ;
Psomas, SK ;
Batsilas, L ;
Paras, SV ;
Kyriakidis, DA ;
Liakopoulou-Kyriakides, M .
PROCESS BIOCHEMISTRY, 2001, 37 (01) :73-80