DISTRIBUTION OF REPETITIVE DNA-SEQUENCES IN EUBACTERIA AND APPLICATION TO FINGERPRINTING OF BACTERIAL GENOMES

被引：2734

作者：

VERSALOVIC, J ^{[1
]}

KOEUTH, T ^{[1
]}

LUPSKI, JR ^{[1
]}

机构：

[1] BAYLOR COLL MED, DEPT PEDIAT, HOUSTON, TX 77030 USA

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 24期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/19.24.6823

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dispersed repetitive DNA sequences have been described recently in eubacteria. To assess the distribution and evolutionary conservation of two distinct prokaryotic repetitive elements, consensus oligonucleotides were used in polymerase chain reaction [PCR] amplification and slot blot hybridization experiments with genomic DNA from diverse eubacterial species. Oligonucleotides matching Repetitive Extragenic Palindromic [REP] elements and Enterobacterial Repetitive Intergenic Consensus [ERIC] sequences were synthesized and tested as opposing PCR primers in the amplification of eubacterial genomic DNA. REP and ERIC consensus oligonucleotides produced clearly resolvable bands by agarose gel electrophoresis following PCR amplification. These band patterns provided unambiguous DNA fingerprints of different eubacterial species and strains. Both REP and ERIC probes hybridized preferentially to genomic DNA from Gram-negative enteric bacteria and related species. Widespread distribution of these repetitive DNA elements in the genomes of various microorganisms should enable rapid identification of bacterial species and strains, and be useful for the analysis of prokaryotic genomes.

引用

页码：6823 / 6831

页数：9

共 60 条

[1] NUCLEOTIDE-SEQUENCE OF THE GLPD GENE ENCODING AEROBIC SN-GLYCEROL 3-PHOSPHATE DEHYDROGENASE OF ESCHERICHIA-COLI K-12 [J].