TARGETED INACTIVATION OF THE INSULIN-RECEPTOR GENE IN MOUSE 3T3-L1 FIBROBLASTS VIA HOMOLOGOUS RECOMBINATION

被引：74
作者：
ACCILI, D [1 ]
TAYLOR, SI [1 ]
机构：
[1] NIDDKD,DIABETES BRANCH,BETHESDA,MD 20892
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 11期
关键词：
TRANSFECTION; ADIPOCYTE DIFFERENTIATION; THYMIDINE KINASE; NEOMYCIN RESISTANCE;
D O I：
10.1073/pnas.88.11.4708
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To study the role of the insulin receptor in determining adipocyte differentiation of the mouse cell line 3T3-L1, we have introduced a mutation that inactivates the insulin receptor gene by homologous recombination. In two independent clones, inactivation of one allele of the insulin receptor gene was associated with a 50-70% reduction in the number of insulin receptors. In addition, both clones were markedly impaired in their ability to differentiate into adipocytes. The defect in adipocyte-specific differentiation was corrected by expression of transfected human insulin receptor cDNA. These data suggest that the insulin receptor may play an important role in promoting differentiation of 3T3-L1 cells into adipocytes in vitro.
引用
收藏
页码：4708 / 4712
页数：5
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