INVITRO SYNTHESIS, GLYCOSYLATION, AND MEMBRANE INSERTION OF INFLUENZA-VIRUS HEMAGGLUTININ

被引:40
作者
ELDER, KT
BYE, JM
SKEHEL, JJ
WATERFIELD, MD
SMITH, AE
机构
[1] IMPERIAL CANC RES FUND,PROT CHEM LAB,LINCOLNS INN FIELDS,LONDON WC2A 3PX,ENGLAND
[2] IMPERIAL CANC RES FUND,TRASLAT LAB,LONDON WC2A 3PX,ENGLAND
[3] NATL INST MED RES,DIV VIROL,LONDON NW7 1AA,ENGLAND
关键词
D O I
10.1016/0042-6822(79)90489-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The synthesis of influenza virus haemagglutinin (HA) has been studied, using cell-free systems primed with cellular RNA from chick embryo fibroblast (CEF) cells infected with influenza virus (A/Japan/305/57-Bellamy/42(H2N1)). In L-cell and wheat germ cell-free systems, a precursor to the HA protein of apparent molecular weight 63,000 was identified by immunoprecipitation and by comparative peptide mapping of this product and the 75,000-dalton glycopolypeptide HA precursor synthesised during infection of CEF cells. The 63,000-dalton precursor was converted to a product of apparent molecular weight 75,000 by addition of dog pancreas membrane vesicles during in vitro translation. The 75,000-dalton protein made in the presence of membranes is protected from trypsin digestion and binds to lectin-Sepharose. These, and further results, suggest that HA is extruded into membrane vesicles and glycosylated during synthesis. © 1979.
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页码:343 / 350
页数:8
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