T-CELL DEPLETION USING ANTI-CD2 COATED MAGNETIC BEADS SIMPLIFIES THE DETECTION OF PERIPHERAL-BLOOD PLASMA-CELLS

被引：17

作者：

WITZIG, TE

GONCHOROFF, NJ

AHMANN, GA

KATZMANN, JA

GREIPP, PR

机构：

[1] Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester

[2] Department of Pathology, State University of New York, Syracuse, NY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 144卷 / 02期

关键词：

MULTIPLE MYELOMA; MAGNETIC BEADS;

D O I：

10.1016/0022-1759(91)90093-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The detection of peripheral blood plasma cells is clinically important, but difficult to perform by use of routine smears. To simplify the detection process, the peripheral blood T cells from ten patients with known active multiple myeloma were depleted using anti-CD2 coated magnetic beads. In all cases, there was enrichment of the immunoglobulin (Ig) positive cells after T cell depletion (mean enrichment factor, 3.4; median, 3.2; range, 1.2-5.1) with a mean pre-bead %Ig+ cells of 7.3 compared to 20.4 for the post-bead sample (p = 0.005). The monoclonal plasma cells were similarly enriched and more easily enumerated on the microscope slides prepared from the T cell depleted sample.

引用

页码：253 / 256

页数：4

共 7 条

[1]

CLOFENT G, 1989, BRIT J HAEMATOL, V71, P357

[2]

DHODAPKAR M V, 1991, Proceedings of the American Association for Cancer Research Annual Meeting, V32, P170

[3] MAGNETIC MONOSIZED POLYMER PARTICLES FOR FAST AND SPECIFIC FRACTIONATION OF HUMAN MONONUCLEAR-CELLS [J].