PRODUCTION AND CHARACTERIZATION OF SPECIFIC BOVINE PLASMINOGEN MONOCLONAL-ANTIBODIES

被引：5

作者：

DUPONT, D

LABIAU, S

GROSCLAUDE, J

COLLIN, JC

机构：

[1] INRA,RECH TECHNOL & ANAL LAITIERES LAB,F-39801 POLIGNY,FRANCE

[2] INRA,VIROL & IMMUNOL MOLEC LAB,F-78352 JOUY EN JOSAS,FRANCE

来源：

HYBRIDOMA | 1994年 / 13卷 / 06期

关键词：

D O I：

10.1089/hyb.1994.13.543

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A collection of monoclonal antibodies was raised against bovine plasminogen by immunizing mice with purified plasminogen. More than 300 positive clones were obtained in 1 fusion experiment (1300 hybrids tested). Four types of antibodies were characterized through their relative binding to plasmin and plasminogen in ELISA. About one-third was strictly specific to plasminogen; the selectivity of this group was confirmed by immunoblots and BIA-core analysis. Cross-reactivity against horse, human, pig, sheep, rabbit, and bovine plasminogens was tested; 41% were strictly bovine specific.

引用

页码：543 / 547

页数：5

共 22 条

[1]

CASTELLINO FJ, 1981, METHOD ENZYMOL, V80, P365

[2]

COLLIN JC, 1988, LAIT, V68, P235, DOI 10.1051/lait:1988216

[3] ESTIMATION OF THE NUMBER OF MONOCLONAL HYBRIDOMAS IN A CELL-FUSION EXPERIMENT - EFFECT OF POST-FUSION CELL DILUTION ON HYBRIDOMA SURVIVAL [J].