THE 3RD ZINC-FINGER OF THE WT1 GENE IS MUTATED IN WILMS-TUMOR BUT NOT IN A BROAD RANGE OF OTHER UROGENITAL TUMORS

Aberrations in the WT1 tumour suppressor gene have been documented in a fraction of Wilms' tumours (WTs). Encoding a protein with four zinc fingers, the WT1 gene is expressed in the developing kidney, gonads, uterus, spleen, mesothelium and brain. Using polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) analysis and direct DNA sequencing, we analysed 156 diverse tumours for abnormalities of zinc finger 3 (ZF3), a mutational hotspot in WT1. Only one sample (WT) exhibited PCR-SSCP mobility shift. A CGA to TGA nonsense mutation at codon 390 with arginine being substituted with a stop codon was detected and predicted to encode a faulty WT1 protein in this WT, out of 8 WTs studied Our results are consistent with the presence of Wt1 ZF3 mutations in a subset of WTs, but not in other tumours of urogenital nor of WT1-related origin.